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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 2988-3005
DOI: https://doi.org/10.20546/ijcmas.2019.804.345


Proteomic Analysis of Cassava Mosaic Virus (CMV) Responsive Proteins in Cassava Leaf
Raghu Duraisamy1*, Senthil Natesan1, Raveendran Muthurajan1, Karthikayan Gandhi2, Pugalendhi Lakshmanan3, Janavi Gnanaguru Janaky3, Nageswari Karuppusamy4 and Mohan Chokkappan5
1Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, India
2Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, India
3Faculty of Horticulture, Tamil Nadu Agricultural University, Coimbatore, India
4Tapioca and Castor Research Station, Tamil Nadu Agricultural University, Yethapur, India
5Central Tuber Crops Research Institute, Trivandrum, Kerala, India
*Corresponding author
Abstract:

Proteomics is becoming an increasingly important tool for the study of many different aspects of plant functions, such as investigating the molecular processes underlying host-pathogen interaction, plant physiology, development and differentiation. Cassava mosaic disease (CMD), caused by cassava mosaic virus (CMV), is the most serious disease in cassava. However, the molecular mechanisms underlying CMD in cassava during CMV infection is not yet clearly understood. The current study determined and identifies the differentially expressed proteins from cassava leaves during the infection of CMV viz., Indian Cassava mosaic virus (ICMV) and Sri Lankan Cassava Mosaic Virus (SLCMV). 2D gel electrophoresis was used to identify the cassava responsive proteins during the virus infection and the differentially expressed proteins were analysed by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. There are 19 proteins were differentially expressed in cassava leaves by CMV infection. Among them 18 were giving good spectra by MALDI-TOF mass spectrometry. Analysis of Peptide Mass Fingerprint (PMF) data of these 18 proteins revealed the identity of the differentially expressed proteins, which suggest their importance and relevance on plant growth and development, and defence. This work paves the way towards a comprehensive analysis of CMV infection of cassava. Identification of the differentially expressed proteins by their sequence homology to known proteins suggests a possible direct or indirect role on plant defence during CMV infection. This study revealed the differentially expressed proteins, expressed during interaction between cassava and CMV that might play important roles either in viral pathogenesis or resistance.


Keywords: Cassava leaf protein, CMV, 2D-PAGE, MALDI-TOF
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How to cite this article:

Raghu Duraisamy, Senthil Natesan, Raveendran Muthurajan, Karthikayan Gandhi, Pugalendhi Lakshmanan, Janavi Gnanaguru Janaky, Nageswari Karuppusamy and Mohan Chokkappan. 2019. Proteomic Analysis of Cassava Mosaic Virus (CMV) Responsive Proteins in Cassava Leaf.Int.J.Curr.Microbiol.App.Sci. 8(4): 2988-3005. doi: https://doi.org/10.20546/ijcmas.2019.804.345