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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 82-94
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.011


Phytochemical Screening, HPTLC and GCMS Profile of Acacia catechu (L.f) Willd Hydroethanolic Leaf Extract
B. Ramesh and V. Jayabharathi
Department of Biochemistry, PSG College of Arts and Science, Coimbatore, India
*Corresponding author
Abstract:

The plant Acacia catechu (L.f) Willd hydroethanolic leaf extract was subjected to qualitative and quantitative phytochemical screening, HPTLC and GCMS analysis. The phytochemical analysis revealed the presence of carbohydrates, proteins, flavonoids, total phenolics, glycosides, total free amino acids, tannins, alkaloids and thiols. The secondary metabolites like flavonoids, tannins and total phenolics were quantified as 82μg/ml, 67.5μg/ml and 42.5μg/ml. The HPTLC analysis of the leaf extract revealed the presence of flavonoids, tannins, phenolics and alkaloids. GCMS chromatogram showed sixty seven peaks which indicated the presence of thirteen phytochemical constituents. The major constituents were Furo[2,3-d] Pyrimidine-4,6 [5H,7H]-dion (10.20%), 2-Methyl-1,2,3,4-tetrahydro-beta-carboline(16.26%), Pthalic acid, butyl 2- pentyl ester (4.35%), Butylphosphonic acid and di(4-methoxy benzene) (14.82%). These results indicated that the Acacia catechu (L.f) Willd contain various bioactive components with wide range of medicinal properties, justifying the use of this plant to treat various diseases.


Keywords: Acacia catechu (L.f) Willd, Hydroethanolic leaf extract, HPTLC, GCMS and Phytoconstituents.
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How to cite this article:

Ramesh, B., and Jayabharathi, V. 2017. Phytochemical Screening, HPTLC and GCMS Profile of Acacia catechu (L.f) Willd Hydroethanolic Leaf Extract.Int.J.Curr.Microbiol.App.Sci. 6(1): 82-94. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.011