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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 2686-2693
DOI: https://doi.org/10.20546/ijcmas.2019.804.312


Assessment of Genetic Variability among the Landraces of Little Millets Panicum sumatrense from Different District of Madhya Pradesh
Lalit Prashad Singh Rajput1, Keerti Tantwai1*, Sajjan Kumar Pooniya1 and Koji Tsuji2
1Biotechnology Centre, Jawaharlal Nehru Agricultural University, Jabalpur - 482 004, Madhya Pradesh, India
2Faculty and Graduate School of Education, Chiba University, Chiba – 263-8522, Japan
*Corresponding author
Abstract:

Present study was conducted on genetic diversity using ISSR markers for a total of 40 landraces of little millet (Panicum sumatrense) collected from five different districts of Madhya Pradesh. Ten ISSR markers amplified total 42 loci while 32 loci showed 76.19% polymorphism. Maximum number (06) of alleles were scored by the primers UBC-807 whereas, minimum number of alleles (03) were scored by the primers UBC-816. Percentage of the number of polymorphic loci within population among the three regions, the highest frequency of polymorphism was found in the Dindori region (97.61) followed by the Betul region (80.95) and the lowest were in the Chhindwara region (40.47). Cluster analysis was estimated and a dendrogram was generated using Unweighted Pair Group Analysis (UPGMA). The highest genetic variability was observed between Amwa-38, Shivri-31 and Khaparipani-24 collected from Rewa and Dindori both of them grouped distantly. The highest PIC value (0.53) was observed by using primer UBC-853 having 06 alleles among the 40 landraces of little millets. The results indicated that ISSR marker system can be effectively used in determination of genetic relationship necessary for their conservation and breeding programs among the landraces of little millets grown in different districts of Madhya Pradesh, India.


Keywords: Polymorphism, UPGMA, Landraces, Genetic variability, Genetic conservation, Phylogenetic relationship
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How to cite this article:

Lalit Prashad Singh Rajput, Keerti Tantwai, Sajjan Kumar Pooniya and Koji Tsuji. 2019. Assessment of Genetic Variability among the Landraces of Little Millets Panicum sumatrense from Different District of Madhya Pradesh.Int.J.Curr.Microbiol.App.Sci. 8(4): 2686-2693. doi: https://doi.org/10.20546/ijcmas.2019.804.312