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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 2045-2053
DOI: https://doi.org/10.20546/ijcmas.2019.804.240


Rising Incidence of Metallo B-Lacatamse Producers in Burns Injury: A Ticking Time Bomb
Sumaira Qayoom Beigh, G.P. Aaftab, M.S. Rashmi* and Muzafar Amin
#333-B, Plot no 16, Behind Govt Urdu HP School, Bhairidevarkoppa, Hubli, Karnataka- 580025, India
*Corresponding author
Abstract:

One of the common causes of morbidity and mortality following burn injury is infections. The emergence of metallo-β-lactamase (MBL) in Gram negative bacilli (GNB) has reduced the available therapeutic options for effective treatment. Therefore, for screening of carbapenemase producers, a simple and inexpensive testing method is essential. To know the prevalence of MBL production in various gram negative isolates, to evaluate different phenotypic methods to detect MBL production and to find out antibiotic sensitivity profile of MBL producing gram negative bacilli. Wound swabs taken from about 100 patients admitted in the burns ward were processed. Kirby Bauer Disc diffusion test was performed on all isolates for antimicrobial susceptibility. Imipenem resistant clinical isolates were taken as positive for MBL screening. Two different methods using EDTA as MBL inhibitor were evaluated: (i) Combined disk synergy test with imipenem (CDST-IPM), (ii) Double-disk synergy test with imipenem (DDST-IPM), according to CLSI guidelines. The most common isolate was Pseudomonas aeruginosa (23%), followed by Staphylococcus aureus (22%), Escherichia coli (14%), Klebsiella pneumoniae (9%), Klebsiella oxytoca (8%),CONS(7%), Proteus mirabilis (5%), Citrobacterfreundii (4%), Citrobacter koseri (3%) and Acinetobacterbaumanii (2%).Out of 82 Gram negative isolates, 17 were resistant to imipenem and were considered screening positive and further tested for MBL production out of which only10 isolates were confirmed as MBL producers. A.baumanii was the predominant MBL producer followed by P.aeruginosa. MRSA rate among S.aureus was 56%, but all were susceptible to glycopeptides and oxazolidinone. Early detection of MBL-carrying organisms, is of paramount clinical importance, as it allows rapid initiation of strict infection control practices as well as therapeutic guidance for confirmed infection.


Keywords: Pseudomonas aeruginosa, Metallo Beta Lactamases (MBLs), Combined Disc Test (CDT), Imipenem -EDTA Double disc Synergy Test (DDST)
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How to cite this article:

Sumaira Qayoom Beigh, G.P. Aaftab, M.S. Rashmi and Muzafar Amin. 2019. Rising Incidence of Metallo B-Lacatamse Producers in Burns Injury: A Ticking Time Bomb.Int.J.Curr.Microbiol.App.Sci. 8(4): 2045-2053. doi: https://doi.org/10.20546/ijcmas.2019.804.240