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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 51-61
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.007


Incidence and Molecular Analysis of Vibrio cholerae among Some Primitive Tribes in Odisha, India

G.P. Chhotray1, M.R. Ranjit1, B.B. Pal1, P.K. Meher2 and H.K. Khuntia1*

1Regional Medical Research Centre, Chandrasekharpur, Bhubaneswar- 751023, India
2Central Institute of Fresh water Aquaculture (CIFA), Kausalya Ganga,Bhubaneswar- 751002, India
*Corresponding author
Abstract:

Cholera has been reported in the state of Odisha, India since more than two decades. The present study was carried out during the year 2000 to 2004 to find out the incidence and molecular analysis of V. cholerae causing diarrhoea among some primitive tribes namely Bondo, Didayi, Juanga and Kondha residing in Odisha, India. Bacteriological analysis of 1066 rectal swabs collected from hospitalized diarrhoea patients in tribal areas revealed 9.8 % were positive for V. cholerae O1 Ogawa and 3.8% for V. cholerae O139 serogroup. The major antibiogram profile of O1 and O139 revealed resistance to ACoFzNNaS and AFzNSNa respectively. The emergence of fluoroquinolone resistant V. cholerae O1 and O139 and 100% nalidixic acid resistant O139 were observed. Cholera toxin production of V. cholerae O1 isolated from the tribal areas exhibited similar to the strains prevail in the endemic coastal districts of Odisha. PCR assay revealed that all the isolates of V. cholerae O1 and O139 were positive for ctxA, tcpA (El Tor), ace and zot genes. Except some minor variation in RAPD profiles, both O1 and O139 strains exhibited similar pattern of PFGE and RAPD among each serogroup compared with the strains of Kolkata and other parts of the state. For the first time, this study revealed that V. cholerae was one of the important aetiopathologic agents responsible for causing diarrhoea in the remote primitive tribal areas in the state, the possible origin and spread of which could be due to the population movement from the endemic areas of the state to the remote tribal areas.


Keywords: Molecular Analysis of Vibrio cholera,Primitive tribes,
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How to cite this article:

Chhotray, G.P., M.R. Ranjit, B.B. Pal, P.K. Meher and Khuntia, H.K. 2016. Incidence and Molecular Analysis of V. cholerae among Some Primitive Tribes in Odisha, India.Int.J.Curr.Microbiol.App.Sci. 6(1): 51-61. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.007