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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 1900-1910
DOI: https://doi.org/10.20546/ijcmas.2019.804.223


Isolation, Pathogenicity and Effect of Different Culture Media on Growth and Sporulation of Alternaria brassicae (berk.) Sacc. causing Alternaria Leaf Spot Disease in Cauliflower
H.T. Valvi, J.J. Kadam and V.R. Bangar*
Department of Plant Pathology, College of Agriculture, Dapoli, India Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, Dist., Ratnagiri- 415 712(M.S.), India
*Corresponding author
Abstract:

The leaf spot disease of Cauliflower (Brassica oleracae L. var. Botrytis) caused by A. brassicae (Berk.) Sacc. was noticed in moderate to severe form on farm of College of Agriculture Engineering and Technology, Dapoli during 2014-2016. The pathogenic fungus was isolated on potato dextrose agar medium from infected leaves of cauliflower. The pathogenicity of the isolated fungus was proved by inoculating healthy seedlings of cauliflower. On the basis of typical symptoms on foliage, microscopic observations and cultural characteristics of the fungus, it was identified as Alternaria spp. The Chief Mycologist, Agharkar Research Institute, Pune identified the pathogenic fungus as Alternaria brassicae (Berk.) Sacc. Eight culture media were tested among that, the potato dextrose agar medium was found most suitable and encouraged maximum radial mycelial growth (90.00 mm) of A. brassicae. The second best culture medium found was host leaf extract agar medium (87.00 mm). This was followed by Richard’s agar medium (75.33 mm) and oat meal agar medium (71.66 mm). Carrot potato agar medium (55.00 mm), Asthana and Hawker’s agar medium (51.66 mm) and Czapek’s Dox agar medium (39.00 mm) were moderate in mycelial growth of A. brassicae. Poor mycelial growth was recorded in V8 juice agar medium (32.66 mm).


Keywords: Cauliflower, Isolation, Pathogenicity, Alternaria brassicae (Berk.) Sacc., Culture media, Sporulation and mycelial growth
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How to cite this article:

Valvi, H.T, J.J. Kadam and Bangar, V.R. 2019. Isolation, Pathogenicity and Effect of Different Culture Media on Growth and Sporulation of Alternaria brassicae (berk.) Sacc. causing Alternaria Leaf Spot Disease in Cauliflower.Int.J.Curr.Microbiol.App.Sci. 8(4): 1900-1910. doi: https://doi.org/10.20546/ijcmas.2019.804.223