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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 1844-1851
DOI: https://doi.org/10.20546/ijcmas.2019.804.215


Effect of Photoperiod on In vitro Culture of Guggul [Commiphora wightii (Arnott)] – A Medicinal Plant
Rajani Verma1*, M.L. Jakhar2 and Ravi Kumar1
Department of Plant Breeding and Genetics, SKN College of Agriculture, Jobner, India
*Corresponding author
Abstract:

Present investigation was carried out the effect of different photoperiod regimes on shoot bud induction, callus induction and shoot regeneration from callus culture in leaf explants of guggul. Standard protocols micropropagation protocol (1.5 mg/l BAP) for nodal segment and (2.0 mg/l Kn) for shoot apex explant and callus induction (2.0 mg/l 2,4-D) and regeneration protocol (1.5 mg/l Kn+ 1.0 mg/l 2,4-D)] were subjected to different photoperiod regimes (16:8, 14:10, 12:12 and 8:16). The cultures were incubated at 25±2°C with a light intensity of 3000 lux. 14:10 hours photoperiod regime was found best for shoot bud induction, callus differentiation and de novo shoot development among all the tested photoperiod regimes.


Keywords: Nodal segment, Shoot apex, Leaf, Photoperiod, Callus, in vitro, Guggul.
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How to cite this article:

Rajani Verma, M.L. Jakhar and Ravi Kumar. 2019. Effect of Photoperiod on In vitro Culture of Guggul [Commiphora wightii (Arnott)] – A Medicinal Plant.Int.J.Curr.Microbiol.App.Sci. 8(4): 1844-1851. doi: https://doi.org/10.20546/ijcmas.2019.804.215