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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(1): 20-28
DOI: http://dx.doi.org/10.20546/ijcmas.2017.601.003


Isolation, Identification and Optimization of Alkaline Amylase Production from Bacillus cereus Using Agro-Industrial Wastes
M. Krishma and S. Radhathirumalaiarasu*
Department of Microbiology, Standard Fireworks Rajaratnam College (Autonomous),Sivakasi- 626 123, Tamilnadu, India
*Corresponding author
Abstract:

Amylase with maximum activity at alkaline pH prefers most industrial applications. The present study investigate amylase production and optimization by bacterial strain isolated and identified from rice mill waste dumped soil. The potent isolate KR9 was identified based on biochemical characteristics and 16S rRNA gene analysis confirmed that this isolate belonged to the genus Bacillus and it was most closely related to B. cereus (99 % similarity). The selected isolate B. cereus KR9 was optimized for pH, growth period and carbon and nitrogen sources.   The highest activity of amylase was obtained at alkaline pH 10.0 after 48 hours (225.2±0.63 U/ml) incubation. Optimization experiment performed with synthetic medium showed starch and peptone as suitable carbon and nitrogen source for amylases production (221±0.77 U/ml).  B. cereus KR9 was further evaluated for amylase production using Solid State Fermentation and Submerged Fermentation with wheat bran, rice straw, paddy straw and sugarcane bagasse. The maximum amylase production of 281.1±0.65 U/ml with specific activity of 1.11±0.01 U/mg was observed with wheat bran using Submerged State Fermentation.  This work has impending utilizing of agro-industrial waste affords cost effective and ecofriendly method for amylase production.


Keywords: Amylase, cereus,alkaline, wheat bran, submerged fermentation.
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How to cite this article:

Krishma, M. and Radhathirumalaiarasu, S. 2017. Isolation, Identification and Optimization of Alkaline Amylase Production from Bacillus cereus Using Agro-Industrial Wastes.Int.J.Curr.Microbiol.App.Sci. 6(1): 20-28. doi: http://dx.doi.org/10.20546/ijcmas.2017.601.003