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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 1639-1647
DOI: https://doi.org/10.20546/ijcmas.2019.804.191


Detection and Characterization of Extended-Spectrum β-lactamases in Salmonella Isolates of Meat, Milk and Human Clinical Samples from Different Districts of Chhattisgarh
Bhoomika1*, Sanjay Shakya1, Anil Patyal1 and Nitin Eknath Gade2
1Department of Veterinary Public Health and Epidemiology
2Department of Veterinary Physiology and Biochemistry, College of Veterinary Science and Animal Husbandry, Chhattisgarh Kamdhenu Vishwa Vidyalaya, Anjora, Durg, Chhattisgarh, India
*Corresponding author
Abstract:

Present study was undertaken to assess the hygiene quality of the meat, milk and also its prevalence on human population. A total of 330 samples including, chicken meat (n=98), Chevon (n=82), raw milk samples (n=90) and human urine (n=56) and stool samples (n=4) Samples were aseptically collected from different districts of Chhattisgarh. Samples were processed for isolation of Salmonella further subjected for the different biochemical tests and confirmed by serotyping. Confirmed isolates were tested for its antibiogram pattern against 13 different antibiotic discs. Isolates were examined for phenotypic method for Extended Spectrum β-lactamases (ESBLs) by double disc synergy test and molecular characterization was done by multiplex PCR method. A total of 14.2% (47/330) samples were positive for Salmonella spp. highest prevalence rate of 27.55% (27/98) were observed in chicken meat, followed by 15% (9/60) and 13.41% (11/82) samples were found positive for Salmonella in contrary none of the milk samples found positive for Salmonella. Serotyping reveals the presence of S. typhiurium and S. enteritidis serotype among the isolates. Isolates showed highest sensitivity (95.7%) against imipenem and however 89.2 % isolates were found resistant to oxytetracylin. Out of 47 isolates 2.12% isolates were found as ESBL positive by phenotypic and genotypic method.


Keywords: ESBL, S. enteritidis, S. typhiurium, Chicken, Chevon
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How to cite this article:

Bhoomika, Sanjay Shakya, Anil Patyal and Nitin Eknath Gade. 2019. Detection and Characterization of Extended-spectrum β-lactamases in Salmonella Isolates of Meat, Milk and Human Clinical Samples from Different Districts of Chhattisgarh.Int.J.Curr.Microbiol.App.Sci. 8(4): 1639-1647. doi: https://doi.org/10.20546/ijcmas.2019.804.191