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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 1578-1590
DOI: https://doi.org/10.20546/ijcmas.2019.804.183


Morphological and Molecular Characterization of Fusarium oxysporum f.sp. Vanilla Inciting Root and Stem Rot Disaease in Vanilla
Mohammed Faisal Peeran1*, Alagupalamuthirsolai Muthalagu1 and C. Sarathambal2
1ICAR-Indian Institute of Spices Research, Regional Station, Appangala, Madikeri – 571 201, Karntaka, India
2ICAR-Indian Institute of Spices Research, Kozhikode-673 012, Kerala, India
*Corresponding author
Abstract:

Vanilla planifolia is popularly known as Prince of Spices a fleshy perennial liana grown in and around Western Ghats for its natural compounds used in several ice creams, chocolates and beverages. Fusarium oxysporum f.sp. vanillae is one of the most destructive pathogens causing severe loss to yield and during the survey conducted in 2016, maximum of 25 % incidence was noticed in coorg district. The pathogen was isolated and morphologically identified as F. oxysporum based on the conidial, chlamydosporial and cultural characters. The size of microconidia ranged between 5.97 to 8.60 µm in to 2.02 to 4.07 µm in width, most of the isolates did not produce macro conidia. Further to confirm the identity of pathogen, 18S rDNA or ITS region DNA was amplified and sequenced. A phylogenetic tree was constructed using Maximum likelihood showed clearly two distinct cluster which clearly out grouped the Colletotrichum gloeosporioides and all other Fusarium sp. in one clade. The seven isolates used in the study grouped under F. oxysporum clearly separating from other species of Fusarium. Futher Tajima’s test also showed only six nucleotide differences indicating that the pathogen is F. oxysporum f.sp. vanilla.


Keywords: Vanilla planifolia, Fusarium oxysporum f.sp. vanilla, Root and stem rot, ITS region, Maximum likelihood
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How to cite this article:

Mohammed Faisal Peeran, Alagupalamuthirsolai Muthalagu and Sarathambal, C. 2019. Morphological and Molecular Characterization of Fusarium oxysporum f.sp. Vanilla Inciting Root and Stem Rot Disaease in Vanilla.Int.J.Curr.Microbiol.App.Sci. 8(4): 1578-1590. doi: https://doi.org/10.20546/ijcmas.2019.804.183