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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 2328-2338
DOI: https://doi.org/10.20546/ijcmas.2019.804.272


Isolation, Identification and Screening of Enterobacter cloacae KY231211 and Brevundimonas aurantiaca KY231210 for Biosurfactant Production
Arjun Karmakar1*, Jyotsna Kiran Peter1, Ankit Singla2 and Anita Raisagar1
1Sam Higginbottom University of Agriculture Technology and Sciences (SHUATS), Prayagraj, Uttar Pradesh, India
2Regional Center of Organic Farming, Ministry of Agriculture & Frames Welfare, Bhubaneswar, Govt. of India
*Corresponding author
Abstract:

In the present study on the basis of cultural, morphological, biochemical and 16s rRNA gene sequencing two rhizospheric bacteria were identified as Brevundimonas aurantiaca KY231210 and Enterobacter cloacae KY231211 and screened for their biosurfactant production potential by oil displacement assay, blood haemolysis assay and emulsification index with vegetable oils (soybean oil and sunflower oil) in different incubation period (0, 24, 48, 72 and 96h). In oil displacement assay, Enterobacter cloacae KY231211 and Brevundimonas aurantiaca KY231210 broth culture revealed positive oil displacement but Brevundimonas aurantiaca KY231210 showed highest displacement values than Enterobacter cloacae KY231211. Both cultures showed haemolysis on blood agar media. Soybean oil showed higher emulsification index with Enterobacter cloacae KY231211 culture while Brevundimonas aurantiaca KY231210 culture showed higher emulsification index with sunflower oil. Brevundimonas aurantiaca KY231210 revealed highest displacement values with Soybean oil. Brevundimonas aurantiaca KY231210 showed highest yield in biosurfactant production as compare to Enterobacter cloacae KY231211.


Keywords: Biosurfactant, Brevundimonas aurantiaca KY231210, Enterobacter cloacae KY231211, PPBS, 16s rRNA
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How to cite this article:

Arjun Karmakar, Jyotsna Kiran Peter, Ankit Singla and Anita Raisagar. 2019. Isolation, Identification and Screening of Enterobacter cloacae KY231211 and Brevundimonas aurantiaca KY231210 for Biosurfactant Production.Int.J.Curr.Microbiol.App.Sci. 8(4): 2328-2338. doi: https://doi.org/10.20546/ijcmas.2019.804.272