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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 791-797
DOI: https://doi.org/10.20546/ijcmas.2019.804.087


Rapid PCR Based Detection of Buffalo Milk in Cow Milk
A.S. Khade*, B.K. Maurya, S.K.J. Ebenezer, S.M. Patel,A. Balakrishnan and Rajesh R. Nair
National Dairy Development Board, Anand, Gujarat, India
*Corresponding author
Abstract:

The study was undertaken with an objective to evaluate a rapid PCR based method for detection of adulteration of buffalo milk in cow milk at minimum level of detection. This method utilizes primers targeting the mitochondrial encoded 12S rRNA gene as the target for species identification. PCR assay involve use of three different primers. Reverse primers specific for cow and buffalo complementary to the gene fragment of 12S rRNA along with the common forward primer. The cow specific primer, along with the common forward primer, yields a cow specific amplicon of 346 bp in the 12S rRNA gene. On the other hand, a buffalo specific primer along with the same common forward primer yields a buffalo specific amplicon of 220 bp fragment in the same gene. The method evaluated was able to detect presence of buffalo milk in cow at 0.5% level of adulteration.


Keywords: Cattle, Buffalo, Milk, Adulteration, PCR
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How to cite this article:

Khade, A.S., B.K. Maurya, S.K.J. Ebenezer, S.M. Patel, A. Balakrishnan and Rajesh R. Nair. 2019. Rapid PCR Based Detection of Buffalo Milk in Cow Milk.Int.J.Curr.Microbiol.App.Sci. 8(4): 791-797. doi: https://doi.org/10.20546/ijcmas.2019.804.087