Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 659-671
DOI: https://doi.org/10.20546/ijcmas.2019.804.071


Amylase Activity of Starch Degrading Bacteria Isolated from Soil
Patel Nimisha1*, Shah Moksha1 and A.K. Gangawane2
1Parul institute of Applied Science & Research, Parul University, P.O. Ghuma, Bopal-Ghuma road, Ahmedabad-380058, Gujarat, India 2Parul institute of Applied science, Parul University, P.O. Limda, Ta. Waghodiya, Dis. Vadodara-390019, Gujarat, India
*Corresponding author
Abstract:

Soil contains huge diversity of microorganism which produces different types of enzymes. Amylase is one of them that hydrolyses starch into its monomer compounds, the smallest being glucose. Hence, amylase is a very prevalent enzyme produced biologically by various kinds of microorganisms and used in industrial sectors for various purposes. Soil bacteria can be isolated and commercially grown in large numbers to produce a vast amount of amylase. In addition, amylases that are extracted require optimum conditions to show greatest activity. In the present study bacteria were isolated from the garden soil and screened for amylase production on starch agar medium. Total 12 isolates were obtained by the primary screening technique from which 05 isolates were showing amylase activity. Zone clearance was determined by Gram’s iodine method. Among 5 isolates isolate 1 was showing highest amylase activity 4.70 U/ml which was considered for further identification. Isolate 1 was tentatively characterized on the basis of their cultural, morphological and biochemical characteristics, which was identified to be Bacillus sp. Further partial purification of the amylase enzyme was carried out by ammonium sulfate precipitation followed by dialysis. Optimization of different parameters was carried out for the amylase production.


Keywords: Amylase, Bacillus subtilis, Soil bacteria
Download this article as Download

How to cite this article:

Patel Nimisha, Shah Moksha and Gangawane, A.K. 2019. Amylase Activity of Starch Degrading Bacteria Isolated from Soil.Int.J.Curr.Microbiol.App.Sci. 8(4): 659-671. doi: https://doi.org/10.20546/ijcmas.2019.804.071