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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 600-605
DOI: https://doi.org/10.20546/ijcmas.2019.804.065


A Study of Metallo-beta-lactamase Producing Pseudomonas aeruginosa Isolates in a Tertiary Care Hospital
Snehal Patil1, Anahita Hodiwala2* and Shailendra Patil1
1BKL Walawalkar Rural Medical College, Sawarde, Chiplun, Maharashtra, India
2MGM Medical College, Maharashtra, India
*Corresponding author
Abstract:

Metallo-beta-lactmase producing Pseudomonas aeruginosa strains are responsible for several nosocomial outbreaks in tertiary care centres across the world. It is well known that poor outcome occurs when patients with serious infections due to MBL producing organisms are treated with antibiotics to which the organism is completely resistant. Therefore, detection of MBL producing Pseudomonas aeruginosa strains is crucial for optimal treatment of critically ill patients and to prevent the spread of resistance. Aim of the present study is to detect Metallo-beta-lactmase (MBL) production in clinical isolates of Pseudomonas aeruginosa by Imipenem-EDTA Double Disc Synergy test. 100 strains of Pseudomonas aeruginosa isolated from pus, sputum, urinary catheter tip, blood and body fluids were screened for Carbapenem resistance by Kirby-Bauer disk diffusion method and results were interpreted as per CLSI guidelines. The isolates showing resistant to Imipenem were further tested for MBL production by Imipenem-EDTA Double Disc Synergy test. Out of 100 Pseudomonas aeruginosa strains 15 were resistant to Imipenem. Out of 15 isolates 10 were MBL producers. Proper antibiotic policy and measures to restrict the indiscriminative use of cephalosporins and carbapenems should be taken to minimize the emergence of Metallo-beta-lactmase producing pathogens.


Keywords: Pseudomonas aeruginosa, Metallo-beta-lactmase, Carbapenem, Multidrug resistance
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How to cite this article:

Snehal Patil, Anahita Hodiwala and Shailendra Patil. 2019. A Study of Metallo-beta-lactamase Producing Pseudomonas aeruginosa Isolates in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 8(4): 600-605. doi: https://doi.org/10.20546/ijcmas.2019.804.065