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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 1004-1011
DOI: https://doi.org/10.20546/ijcmas.2019.804.116


Analysis of Gonadotropin Releasing Hormone (GnRH), Leutinizing Hormone (LHb) and Follicle Stimulating Hormone (FSHb) Genes in Indian Spiny Loach (Lepidocephalus thermalis)
R. Hamsavalli1*, J. Jaculine Pereira1, K. Karal Marxand R. Jeya Shakila2
1Department of Fisheries Biotechnology,
2Department of Fish Quality Assurance and Management, Fisheries College and Research Institute, Thoothukudi, India
3Institute of post graduate study, Tamil Nadu Dr. J. Jayalalithaa Fisheries University, OMR campus, Chennai, India
*Corresponding author
Abstract:

Lepidocephalus thermalis forms a potential candidate species for aquaculture, being a native species it also has high market demand with easy domestication procedure. RNA was extracted from the ovarian tissues of Lepidocephalus thermalis by following the TRIZOL reagent method and cDNA was synthesized. The purity of RNA, cDNA and PCR products were checked. The PCR reactions were carried out with gene specific primers of gonadotropin releasing hormone II, gonadotropin releasing hormone III, leutinizing hormone and follicle stimulating hormone genes and the PCR products size of the four genes were 270-bp for gonadotropin releasing hormone II, 250-bp for gonadotropin releasing hormone III, 290-bp for leutinizing hormone and 260-bp for follicle stimulating hormone. The results from present study will serve as a primary data for further research and development in the reproductive genomic aspects of Indian spiny loach (Lepidocephalus thermalis).


Keywords: Lepidocephalusthermalis, Gonadotropin releasing hormone, Follicle stimulating hormone and leutinizing hormone
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How to cite this article:

Hamsavalli, R., J. Jaculine Pereira, K. Karal Marx and Jeya Shakila, R. 2019. Analysis of Gonadotropin Releasing Hormone (GnRH), Leutinizing Hormone (LHb) and Follicle Stimulating Hormone (FSHb) Genes in Indian Spiny Loach (Lepidocephalus thermalis).Int.J.Curr.Microbiol.App.Sci. 8(4): 1004-1011. doi: https://doi.org/10.20546/ijcmas.2019.804.116