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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 795-818
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.090


Efficacy of Zinc Oxide Nanoparticles and Curcumin in Amelioration the Toxic Effects in Aflatoxicated Rabbits
Hassan A. Atef1*, Mogda K. Mansour2, Essam M. Ibrahim3, Rasha M.H. Sayed El-Ahl1, Naglaa M. Al-Kalamawey3, Y.A. El Kattan4 and M.A. Ali4
1Department of Mycology and Mycotoxins, Animal Health Research Institute, Agriculture Research Centre, Dokki, Cairo, Egypt
    2Department of Biochemistry, Toxicology and Feed Deficiency, Animal Health Research Institute, Agriculture Research Centre, Dokki, Cairo, Egypt
3Department of Pathology, Animal Health Research Institute, Agriculture Research Centre, Dokki, Cairo, Egypt
4Department of Biochemistry, Regional Laboratory at Minufiya Governorate, Egypt
*Corresponding author
Abstract:

As the first step in this direction; biosynthesis and characterization of zinc oxide nanoparticles (ZnO-NPs) and their use to ameliorate the toxic effects of aflatoxicosis in rabbit in comparison with curcumin were studied. Thirty-five healthy New Zealand rabbits were divided into 5 groups, each including 7 rabbits. Group 1 was kept as negative control without any treatment. Rabbits in the groups 2, 3, 4 and 5 were given AFB1 orally at a dose of 50 ug dissolved in 0.5 ml of olive oil/ animal daily, for 4 weeks. On day one following the administration of AFB1, curcumin was orally administered at a dose of 15 mg/kg b.w. for group 3. While, animals of Group 4, were orally administered AFB1 +   ZnO-NPs  (25   ug  / kg of B.W. of animal /0.2 mL of buffer/day)( low dose). Animals of Group 5, were orally administered aflatoxins +   ZnO- NPs (50 ug  / kg of B.W. of animal /0.2 mL of buffer/day) ( high dose). The biochemical evaluation of rabbit sera after experimental study showed that AFB1 increased the concentration of NO and MDA, while decreased the level of GSH and activities of SOD, CAT and GSH-PX. Significant decreased values in plasma total protein, albumin, alpha globulin, beta globulin, and gamma globulin. Decrease in serum globulin in toxin fed group might be due to the adverse effect of aflatoxin B1 on synthesis of total proteins and globulin. The AFB1 had a drastic effect on liver and kidneys with marked genotoxic effect and inhibition of DNA, RNA and protein synthesis. These changes were improved by administration of ZnO-NPs or curcumin to aflatoxicated animal through the immune strengthening effect and protection of lipid and protein from oxidative damage. It is concluded that the addition of ZnO-NPs and curcumin to animal feed possesses hepato-protective effect through scavenging of free radicals, or by enhancing the activity of antioxidant, which then detoxify the free radicals. These factors protect hepatic cells from the damaging effect by AFB1 that could be progress to hepatocarcinmoa.


Keywords: Aflatoxicosis,Rabbits, ZnO-Nps, curcumin, GSH, Hepatoprotective effect, anti-hepatocellular carcinoma, Antioxidant, Fibrosis, Apoptosis.
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How to cite this article:

Hassan A. Atef, Mogda K. Mansour, Essam M. Ibrahim, Rasha M.H. Sayed El-Ahl, Naglaa M. Al-Kalamawey, Y.A. El Kattan and M.A. Ali. 2016. Efficacy of Zinc Oxide Nanoparticles and Curcumin in Amelioration the Toxic Effects in Aflatoxicated Rabbits.Int.J.Curr.Microbiol.App.Sci. 5(12): 795-818. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.090