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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 913-919
DOI: https://doi.org/10.20546/ijcmas.2019.804.105


Molecular Investigation of Rifampicin Resistance in Clinical Isolates of Mycobacterium ulcerans in Côte D'ivoire
Bakary Coulibaly1*, David N’golo Coulibaly2, Clément Kouassi Kouassi1, Ibrahim Konaté1, Elise Solange Ngazoa-Kakou2 and Mireille Dosso2
1UFR Agroforestry, Agrovalorization Laboratory, Department of Biochemistry Microbiology, Jean Lorougnon Guédé University, BP 150 Daloa, Ivory Coast
2Molecular Biology Platform, Institut Pasteur Côte d'Ivoire, BP 490 Abidjan, Ivory Coast
*Corresponding author
Abstract:

M. ulcerans is the etiologic agent of Buruli ulcer. The clinical diagnosis of infection with this mycobacterium is based on microbiological analyzes, including PCR, which uses the nucleotide sequences of the primers to detect the targeted sequences. Cases of rifampicin resistance were recorded in mice experimentally infected with M. ulcerans strains and treated with rifampicin in previous studies. In this study, clinical isolates were confirmed as M. ulcerans strains. These isolates are from samples of patients with Buruli ulcer. The PCR gave a positivity rate of 92.10% for the detection of the IS 2404 sequence and made it possible to highlight the virulence gene in most of the strains studied with a positivity rate of 94.74% for the detection of the sequence IS-KR. However, the RpoB gene could not be found in any of the strains. Thus it gives a positivity rate of 0% for the detection of this gene. The results show that most strains of M. ulcerans secrete mycolactone. The production of this toxin is the consequence of a mutation in the IS-KR gene. They also show that rifampicin has an effective bactericidal activity against M. ulcerans strains and that resistance to this antibiotic results from a mutation of the RpoB gene.


Keywords: M. ulcerans, PCR, IS2404, Buruli ulcer, Ivory Coast, RpoB gene, ISKR sequence
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How to cite this article:

Bakary Coulibaly, David N’golo Coulibaly, Clément Kouassi Kouassi, Ibrahim Konaté, Elise Solange Ngazoa-Kakou and Mireille Dosso. 2019. Molecular Investigation of Rifampicin Resistance in Clinical Isolates of Mycobacterium ulcerans in Côte D'ivoire.Int.J.Curr.Microbiol.App.Sci. 8(4): 913-919. doi: https://doi.org/10.20546/ijcmas.2019.804.105