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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(4): 309-324
DOI: https://doi.org/10.20546/ijcmas.2019.804.035


Psp68, A Dead Box Helicase Confers Salinity Tolerance in Transgenic Pigeon Pea
Neha and Pushpa Kharb*
College of Basic Sciences and humanities, CCS HAU, Hisar, Haryana, India
*Corresponding author
Abstract:

Legumes are an important part of human diet account for about 27% of global primary crop production. Pigeon pea is the world’s sixth most important and second most important legume pulse crop of India after chickpea and mainly cultivated as rain fed crop. Its production is adversely affected due to salinity in arid and semi-arid regions of world. Salt stress reduces water potential, creates imbalance in ion concentration and causes toxicity. Helicases have been shown to play an important role in plants against salt stress. p68 which is a prototype member of DEAD-box helicase interacts with Ca2+-CaM, thus regulating diverse signalling pathways against salt stress in plants. In the present study, we have developed transgenic pigeon pea plants with marker free gene Psp68 for salinity tolerance. Since regeneration is prerequisite for transgenic development and pigeon pea is considered to be recalcitrant, the transgenic pigeon pea plants containing Psp68 gene have been developed using the tissue culture independent transformation method (Patent Application No. 201811012099). The putative T0 plants were screened by PCR analysis and the PCR positive plants with transformation efficiency of 16% were observed. Transgenic lines in T1 generation under salt stress condition showed enhanced tolerance to salt stress in terms of various physio-biochemical parameters like relative water content, membrane injury index, MDA content, chlorophyll content, proline and total soluble sugar content, catalase activity and peroxidase activity.


Keywords: Agrobacterium Transformation, Transgenic, Physio-biochemical analysis
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How to cite this article:

Neha and Pushpa Kharb. 2019. Psp68, A Dead Box Helicase Confers Salinity Tolerance in Transgenic Pigeon Pea.Int.J.Curr.Microbiol.App.Sci. 8(4): 309-324. doi: https://doi.org/10.20546/ijcmas.2019.804.035