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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 3454-3458
DOI: https://doi.org/10.20546/ijcmas.2019.802.404


Analysis of GC-content in transcriptome sequence of Coscinium fenestratum (Gaertn.) Colebr Leaf
Ashalatha and S. M. Gopinath*
Department of Biotechnology, Acharya Institute of Technology, Bengaluru, Karnataka, India-560107
*Corresponding author
Abstract:

Coscinium fenestratum (Gaertn.) Colebr, a important medicinal vine is considered as critically endangered or restricted to the humus rich soil. It has abundant use in ayurvedic, siddha, tibetan medicine system. The plant is already Red listed and at the verge of extinction. So a transcriptome study and the GC analysis of the plant are vital. The research provides information on its transcriptome and its stability which can be used for further studies. The leaf tissue of C.fenestratum was collected, sequenced using illumina paired end sequencing. The raw sequence data quality check parameters like the average base content and the GC content of the reads were analyzed. Maximum number of reads showed 43% of the average GC content in the sample showing slightly instability to adaptation.


Keywords: Guanine, Cytosine, Illumina paired end sequencing
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How to cite this article:

shalatha and Gopinath, S. M. 2019. Analysis of GC-content in transcriptome sequence of Coscinium fenestratum (Gaertn.) Colebr Leaf.Int.J.Curr.Microbiol.App.Sci. 8(2): 3454-3458. doi: https://doi.org/10.20546/ijcmas.2019.802.404