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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 3449-3453
DOI: https://doi.org/10.20546/ijcmas.2019.802.403


Microscopic Colonization of Salt Tolerant Plant–Bacterium Microvirga zambiensis Strain WSM 3693 in Cotton Tissue using Auto Fluorescent Proteins GFP
R.A. Bhadania, M.V. Parkhiya, D.L. Akbari, L.F. Akbari, H.N. Bhalani and B.A. Golakiya
Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh 362001, India
*Corresponding author
Abstract:

The Microvirga zambiensis strain WSM 3693 is an endophytic strain isolated from saline area of kutch, Gujarat. The green fluorescent protein (GFP) was used to mark this endophyte in order to visualize and analyze the colonization processes in vivo. After inoculation for 2 to 15 d, it was visualized in cotton tissue using fluorescence microscopy. Thus, the objectives of this study were to construct gfp-marked derivatives of Microvirga zambiensis strain WSM 3693and to determine the endophytic patterns of colonization of cotton under gnotobiotic conditions by using genetically marked derivatives of this strain.


Keywords: Microvirga zambiensis strain WSM 3693, Endophyte, GFP, Cotton
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How to cite this article:

Bhadania, R.A., M.V. Parkhiya, D.L. Akbari, L.F. Akbari, H.N. Bhalani and Golakiya, B.A. Microscopic Colonization of Salt Tolerant Plant–Bacterium Microvirga zambiensis Strain WSM 3693 in Cotton Tissue using Auto Fluorescent Proteins GFP.Int.J.Curr.Microbiol.App.Sci. 8(2): 3449-3453. doi: https://doi.org/10.20546/ijcmas.2019.802.403