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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 726-732
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.083


Molecular Detection of Citrus Yellow Mosaic Virus (CYMV) and Citrus Greening Bacterium (CGB) in Sathgudi Sweet Orange by Duplex Polymerase Chain Reaction (dPCR)
V. Gopi1, T. Gouri Sankar1, K. Gopal2* and L. Mukunda Lakshmi1
1Molecular Diagnosis Laboratory, Citrus Research Station, Dr. Y.S.R. Horticultural University,
Tirupati – 517 502, Andhra Pradesh, India
2College of Horticulture, Dr. Y.S.R. Horticultural University, Anantharajupeta – 516 105, Andhra Pradesh, India
*Corresponding author
Abstract:

Citrus is one of the most economically important fruit crop in India. Commercially grown citrus group in India includes sweet orange, acid lime and mandarin. Various factors constraint fruit production globally but the major factor is diseases caused by fungal, bacterial and viral pathogens. Citrus yellow mosaic virus (CYMV), a viral disease and Citrus greening bacterium (CGB), a bacterial disease are the two most important diseases that are impending fruit production throughout the world. The combined incidence of the diseases in Andhra Pradesh was found to be 32 to 68%.  A rapid and reliable duplex PCR is developed for the simultaneous detection of CYMV and CGB in Sathgudi sweet orange plants. Initially total DNA was isolated from mixed infected field samples for both pathogens were detected simultaneously through duplex PCR. Using duplex PCR two different fragments of 726 bp and 451 bp specific to CYMV and CGB respectively were simultaneously amplified. The consistent result of duplex PCR was compared with simplex PCR for detection of each pathogen. The duplex PCR method developed in the present investigation proved to be highly sensitive, economic and reliable method for detection of both CYMV and CGB in citrus pants. The technique would also prove highly useful in disease survey, nursery certification and quarantine applications.


Keywords: Citrus Yellow Mosaic Virus (CYMV), Citrus Greening Bacterium (CGB), Duplex PCR, Simultaneous Detection.
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How to cite this article:

Gopi, V., T. Gouri Sankar, K. Gopal and Mukunda Lakshmi, L. 2016. Molecular Detection of Citrus Yellow Mosaic Virus (CYMV) and Citrus Greening Bacterium (CGB) in Sathgudi Sweet Orange by Duplex Polymerase Chain Reaction (dPCR).Int.J.Curr.Microbiol.App.Sci. 5(12): 726-732. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.083