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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 2464-2469
DOI: https://doi.org/10.20546/ijcmas.2019.803.291


In vitro Shoot Proliferation from Nodal Segments of Indian Blackberry (Syzygium cumini L.)
Vividha M. Sirsat, Darasing R. Rathod, Santosh J. Gahukar, Amrapali A. Akhare, Vijay L. Gawande, Neha C. Maske, Balwant S. Munde, Dipika A. Padole and Aditya V. Rathod
Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola, Dist. Akola (M.S.), India
*Corresponding author
Abstract:

Micropropagation of Indian Blackberry (Syzygium cumini L.) species locally called as Jamun, has always been a challenging task. In India, Jamun tree is an underutilized minor fruit crop species possessing a high medicinal and nutritional value. An efficient protocol for rapid shoot proliferation of Jamun (cv. Rajamun) from nodal segment of locally grown mature trees has been developed. Experiments were conducted at Biotech Centre, Dr. PDKV, Akola during 2016-18. The Lloyd and McCown woody plant medium (WPM) used as a basal media supplemented with growth regulators at varying concentrations. Nodal explants were cultured on WPM supplemented with BAP (6-Benzylamino purine 8, 8.5 and 9 µM/l), Kn (Kn 8, 8.5 and 9 µM/l). WPM supplemented with BAP + Kn @ 8 µM/l was proved to be significantly best at 5% level for high survival rate of 93.33% and required less time i.e. 32.43 days to initial shoot sprout after explants inoculated with 2.53 initial shoot multiples. The treatments for shoot proliferation were conducted on WPM supplemented with BAP @ 13, 14 and 15 µM/l, Kn @ 8, 8.5 and 9 µM/l and NAA (Naphthalene acetic acid) @ 5 µM/l. For high frequency multiple shoot induction WPM with 13µM BAP + 8.5µM Kn + 5µM NAA was proved to be significantly superior at 5% level after 4 weeks of first subculture giving 2.87 shoot multiples, followed by maximum 4.20 shoot multiples at 6 weeks stage of second subculture with significantly 3.21cm shoot length elongation at 10 weeks when supplemented on same media against 1 cm of control.


Keywords: in vitro, Indian blackberry, Jamun, Micropropagation, Nodal segment, Proliferation, Syzygium cumini L., Woody plant media
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How to cite this article:

Vividha M. Sirsat, Darasing R. Rathod, Santosh J. Gahukar, Amrapali A. Akhare, Vijay L. Gawande, Neha C. Maske, Balwant S. Munde, Dipika A. Padole and Aditya V. Rathod. 2019. In vitro Shoot Proliferation from Nodal Segments of Indian Blackberry (Syzygium cumini L.).Int.J.Curr.Microbiol.App.Sci. 8(3): 2464-2469. doi: https://doi.org/10.20546/ijcmas.2019.803.291