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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 2386-2398
DOI: https://doi.org/10.20546/ijcmas.2019.803.282


Interaction between some Plant Extracts with some Antibiotics against Staphylococcus aureus from Chickens
Ashraf, A. Abd-El Tawab1, Ahmed A. Ammar2, Ahmed M. Hamouda3, Wafaa A. EL Sebaey4, Salma S. El-Deen4 and Safinaz A.M. Elhawary5*
1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Benha University, Moshtoher, Qalyubiyagovernate, Egypt
2Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Sharkiagovernate, Egypt
3Animal Health Research Institute Zagazig, Sharkiagovernate, Egypt
4Animal Health Research, Dokki, Giza, Egypt
5Faculty of Veterinary Medicine, Benha University, Qalyubiyagovernate, Egypt
*Corresponding author
Abstract:

Multidrug-resistant bacterial strains are becoming a serious problem. Therefore, the application of natural antimicrobial agents from plant extracts combined with antibiotics to overcome this problem is of major importance. The antimicrobial activity of five plants(geranium, turmeric, thyme, ginger and green tea)methanol extract prepared by ultrasonic-assisted (UAE) combined with antibiotics (amoxicillin, doxycycline, gentamicin and difloxacin) against 16 Staphylococcus aureus chickens isolates was tested using in vitro methods. The interactions between plant extracts and antibiotics are known to be either additive or synergistic or antagonistic. The mean zones of inhibition (mm) and the minimum inhibitory concentration (MIC) of plant extracts and of antibiotics and combination between them was determined. The total phenolic content (TPC) and the antioxidant activity (DPPH) of plant extracts were evaluated. Methanol extracts had high total phenolic compounds which used as a source of natural antioxidants. The results revealed that synergistic effects appear thyme with amoxicillin and gentamicin and difloxacin. Turmeric had synergistic effect with amoxicillin, doxycycline, and difloxacin. Synergistic activity against Gram-positive bacteria demonstrated that extracts could be a source of bioactive substances with a broad spectrum of antibacterial activity especially when combined with antibiotics. In addition, extracts are potential safe sources of bioactive compounds, antioxidants, antibacterial agents which might be applied in different foods and pharmaceutical products.


Keywords: Decimal Assay for Additivity (DAA), Antiradical, Antimicrobial, Amoxicillin, Doxycycline, Gentamicin, Difloxacin
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How to cite this article:

Ashraf, A. Abd-El Tawab, Ahmed A. Ammar, Ahmed M. Hamouda, Wafaa A. EL Sebaey, Salma S. El-Deen and Safinaz A.M. Elhawary. 2019. Interaction between some Plant Extracts with some Antibiotics against Staphylococcus aureus from Chickens.Int.J.Curr.Microbiol.App.Sci. 8(3): 2386-2398. doi: https://doi.org/10.20546/ijcmas.2019.803.282