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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 710-717
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.081


Evaluation of NS1 Antigen Detection for Early Diagnosis of Dengue Virus Infection in a Tertiary Care Hospital in Karnataka, India
Rajani Ranganath1* and Basavaraj V. Peerapur2
Department of Microbiology, Raichur Institute of Medical Sciences, Raichur, Karnataka, India
*Corresponding author
Abstract:

To evaluate the efficacy of NS1 antigen as a early marker for diagnosis of Dengue virus infection.  Performance of NS1 antigen assay in comparison to MAC ELISA was evaluated when performed together in a single sample in Group 1. 391 acute/early convalescent sera were screened by both the assays. Group 2 evaluated the specificity of NS1 assay in comparison to MAC ELISA on 30 samples included as controls. In Group 1, 162(41.43%) were positive by NS1 antigen assay and 71(18.15%) were positive by MAC ELISA. NS1 antigen positivity decreased from 96.91% in acute phase sera to 3.08% in early convalescent sera (< 0.0001). Conversely IgM positivity increased from 7.04% in acute phase sera to 92.95% in early convalescent sera (< 0.0001). All the samples in Group 2 were negative showing 100% specificity of both the assays.  NS1 Ag is an effective tool for the diagnosis of DENV infection, especially with in first 7 days of illness. Combined use of NS1 antigen assay with MAC ELISA test could significantly improve diagnostic sensitivity of dengue infection.


Keywords: Dengue diagnosis, NS1 antigen assay, MAC ELISA, acute phase, convalescent phase.
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How to cite this article:

Rajani Ranganath and Basavaraj V. Peerapur. 2016. Evaluation of NS1 Antigen Detection for Early Diagnosis of Dengue Virus Infection in a Tertiary Care Hospital in Karnataka, India.Int.J.Curr.Microbiol.App.Sci. 5(12): 710-717. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.081