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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 2322-2328
DOI: https://doi.org/10.20546/ijcmas.2019.803.275


Antibiogram and Biofilm Phenotypic Characterization of E. coli Isolates from Milk and Environmental Sources
Bobade Sumedha*, R.M. Gade, S. Rajurkar, A. Raut, P. Uike and A. Bhoyar
Vasantrao Naik College of Agricultural Biotechnology, Yavatmal (M.S.), India
*Corresponding author
Abstract:

Escherichia coli is responsible for a wide variety of intestinal infections, showing increasing antimicrobial resistance. Antimicrobials resistance in biofilm-forming isolates contributes to bacterial persistence which may lead major public health concern and treatment problems. The aim of this study was to study the antimicrobial resistance profile of E. coli with reference to biofilm production to study the possible relationship among E. coli isolates from cattle and their farm environment. Out of 64 samples 34 (53.12%) samples were confirmed as E. coli, whereas 16 (47.05%) isolates were found to be Biofilm producer on Congo red Agar. The recovered isolates (18) were further studied for Antibiotics sensitivity patterns against 6 antibiotics. The highest number of isolates was resistance to Tetracycline (66%) and Ampicillin (66%). The isolates were susceptible to other antibiotics like Chloramphenicol (77.78%), Ciproflloxacin (77.78%), Streptomycin (88.89%). All isolates were sensitive to Gentamycin. The different Antibiotic resistivity patterns have been observed among the isolates. E. coli is an indication of poor hygienic practices in dairy. These organisms originate from the cow's environment and infect the udder may enter the food chain by faecal contamination and pose potential public health hazards. Biofilm production by these pathogenic organism make resistant to antibiotics and there is possibility of public health threat from such drug resistance strains of E. coli.


Keywords: Antibiogram, Antibiotic, Biofilm, Environment, Resistance
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How to cite this article:

Bobade Sumedha, R.M. Gade, S. Rajurkar, A. Raut, P. Uike and Bhoyar, A. 2019. Antibiogram and Biofilm Phenotypic Characterization of E. coli Isolates from Milk and Environmental Sources.Int.J.Curr.Microbiol.App.Sci. 8(3): 2322-2328. doi: https://doi.org/10.20546/ijcmas.2019.803.275