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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 2166-2175
DOI: https://doi.org/10.20546/ijcmas.2019.803.259


Effect of Different Spore Concentration of Entomopathogenic Fungi Isolates against Mealy Bug Maconellicocus hirsutus
S.B. Sable1*, P.B. Deore1, V.R. Bangar2 and K.C. Kurhade2
1College of Agriculture, Dhule, Plant Pathology Section, MPKV, Rahuri, Maharashtra, India
2Department of Plant Pathology, PGI, MPKV, Rahuri, Maharashtra, India
*Corresponding author
Abstract:

Entomopathogenic fungi suppress the diverse group of insect pest such as coleopterans, lepidopterous and sucking pest. Wide spread incidence of Aspergillus niger, Aspergillus flavus and Aspergillus tamarii was observed during extensive and repeated survey covering different agro ecological niches of Dhule, Nandurbar, Jalgaon, Nasik and Beed districts of Maharashtra (India) on insect cadavers infected with fungi. From the insect cadavers the fungal isolates were isolated on PDA media. Out of nineteen samples collected only fifteen samples showed the growth of fungus on DOC 2-50% selective media. Virulence of entomopathogenic fungi isolates against mealy bugs (Maconellicoccus hirsutus) revealed that as the spore concentration increased from 10-3 to 10-9 the insect mortality was decreased. Among all the fifteen isolates the highest insect mortality was recorded at 10-3 spores/ml concentration and the spore dilution of entomopathogenic fungi isolates increased from 10-3 to 10-9 spores/ml the time required for insect mortality was also increased. Among the isolates of entomopathogenic fungi the isolate EPF-14 was the most virulent. Among each isolate, it was observed that as the spore dilution increased the insect mortality decreased. Aspergillus tamarii, A. niger and A. flavus the isolate EPF-7, EPF-14 and EPF-6 were the most virulent, respectively against mealy bugs at 10-3 spores/ml concentration and isolates EPF-1, EPF-2 and EPF-6 were the most virulent isolates respectively against mealy bugs at 10-6 and 10-9 spores/ml concentration.


Keywords: Entomopathogenic fungi, Cadaver, Aspergillus Spp., Isolates, Virulent, Concentration, Maconellicoccus hirsutus
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How to cite this article:

Sable, S.B., P.B. Deore, V.R. Bangar and Kurhade, K.C. 2019 Effect of Different Spore Concentration of Entomopathogenic Fungi Isolates against Mealy Bug Maconellicocus hirsutus.Int.J.Curr.Microbiol.App.Sci. 8(3): 2166-2175. doi: https://doi.org/10.20546/ijcmas.2019.803.259