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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 2156-2165
DOI: https://doi.org/10.20546/ijcmas.2019.803.258


Variability in Morphology and Growth Characteristics of Different Isolates of Entomopathogenic Fungi Managing the Mealy Bugs Maconellicocus hirsutus
S.B. Sable1*, P.B. Deore1, H.V. Deshmukh2, H.N. Markad2 and G.B. Jejurkar2
1College of Agricultur, Dhule, Plant Pathology Section, MPKV, Rahuri, Maharashtra, India
2Department of Plant Pathology, PGI, MPKV, Rahuri, Maharashtra, India
*Corresponding author
Abstract:

Current research efforts are directed towards native entomopathogenic fungi which are highly virulent to insect pests to develop efficient and eco-friendly bio-pesticides. From the insect cadavers fifteen different fungal isolates were isolated on DOC2-50% selective media and were identifying as isolates of Aspergillus tamari, A. niger and A. flavus. All the fifteen isolates showed variation in all the morphological characters studied. Highest mean colony diameter (mm) was reported in isolate EPF-14 at all the time intervals. The lowest mean colony diameter (mm) was reported in isolate EPF-13 at 24, 72 and 96 hr interval while at 48 hrs the lowest mean colony diameter (mm) was reported in isolate EPF-9. The most of the isolates were not produced any colony pigmentation on PDA media. The isolates EPF-12 and EPF-15 were grayish green color, while EPF-1 and EPF-7 observed light grayish green color. The isolate EPF-5 was dark grayish green color while, EPF-13 was yellowish grayish green color. The isolates EPF-9 & EPF-11 were dull whitish green color and isolate EPF-6 & EPF-8 were dark green and bluish green color respectively. The isolates EPF-2 & EPF-14 were black in color while, isolates EPF-4, EPF-3 & EPF-10 were dark black, light black and bluish black in color respectively. Among all the isolates, the isolates EPF-1, EPF-4, EPF-5, EPF-6, EPF-7, EPF-9, EPF-11, EPF-12, EPF-13 & EPF-15 produced the concentric rings while, in isolate EPF-2, EPF-3, EPF-8, EPF-10 & EPF-14 concentric rings were absent. Isolates showed variation in the spore’s shape, size and colours. The spore shape was varying from round to globose. While, spore size was varying from to 10.1 x 9.7 = 97.97µ to 4.3 x 4.2 = 18.06 µ and length width ratio varies from 1.06 to 1.00. The colour of spores was varies from brown to yellow except in isolate EPF-1, EPF-11 and EPF-13.


Keywords: Entomopathogenic fungi, Cadaver, Aspergillus spp., Colony diameter, Spores shape, PDA media, Maconellicoccus hirsutus
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How to cite this article:

Sable, S.B., P.B. Deore, H.V. Deshmukh, H.N. Markad and Jejurkar, G.B. 2019. Variability in Morphology and Growth Characteristics of Different Isolates of Entomopathogenic Fungi Managing the Mealy Bugs Maconellicocus hirsutus.Int.J.Curr.Microbiol.App.Sci. 8(3): 2156-2165. doi: https://doi.org/10.20546/ijcmas.2019.803.258