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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 1910-1918
DOI: https://doi.org/10.20546/ijcmas.2019.803.227


Isolation and Characterization of Rhizobium Associated with Root Nodules of Dalbergia sissoo
Meenakshi Dhiman*, Vinay Kumar Dhiman, Neerja Rana and Bhawna Dipta
1Microbiology Division
2Department of Basic Sciences, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan-173230, Himachal Pradesh, India
*Corresponding author
Abstract:

A study was conducted to isolate and characterize the rhizobia from the root nodule of Dalbergia sissoo from five different sites of Himachal Pradesh (Bilaspur, Solan, Hamirpur, Kangra and Una) and Uttrakhand (Rishikesh, Nainital, Haridwar, Dehradun and Udham Singh Nagar). A total of 79 bacterial isolates were isolated from root nodule of Dalbergia sissoo on YEMA media after 3 days of incubation at 37˚C by serial dilution method. Out of 79 isolates only 38 isolates were identified as rhizobia on the basis of authentication test (Congo red test, Bromothymol blue test and Plant infection test). Thirty eight strains were characterized on the basis of biochemical characterization. On the basis of morphological properties all the isolates were round and slimy white with raised elevation and smooth surface indicating rhizobia. In Gram’s reaction they were pink, rod in shape indicating gram negative bacteria. Only two rhizobial isolates DBD1 and DRT5 were selected on the basis of plant growth promoting traits (P-solubilization, Nitrogen fixation, Siderophore production, HCN production and Biocontrol activity). These two rhizobial isolates may be useful to increase the symbiotic nitrogen fixation in legume tree and can be used as potential biofertilizer owing to their plant growth promoting characters.


Keywords: Isolation, Characterization, Rhizobia, Biofertilizers, Nitrogen fixation
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How to cite this article:

Meenakshi Dhiman, Vinay Kumar Dhiman, Neerja Rana and Bhawna Dipta. 2019. Isolation and Characterization of Rhizobium Associated with Root Nodules of Dalbergia sissoo.Int.J.Curr.Microbiol.App.Sci. 8(3): 1910-1918. doi: https://doi.org/10.20546/ijcmas.2019.803.227