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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 1688-1696
DOI: https://doi.org/10.20546/ijcmas.2019.803.196


Characterization of Rhizosphere Soils of Foxtail Millet and Isolation of Azospirillum Strains
N.R. Vijayalakshmi1* and Mahadeva Swamy2
1Department of Agricultural Microbiology, College of Agriculture, Raichur, India
2University of Agricultural Sciences, Raichur-584101, Karnataka, India
*Corresponding author
Abstract:

A Total of 40 different foxtail millet roots samples along with rhizospheric soil were collected from Raichur and Koppal districts of Karnataka. Azospirillum strains were isolated and physic-chemical properties of the soil were analyzed. Among them, 20 samples each from Raichur and Koppal districts were collected. The physico-chemical properties of the soil and available nutrients of Raichur district samples ranged from, pH (7.58-8.85), electrical conductivity (0.12-0.99 dS m-1), organic carbon (0.11-0.87%), available nitrogen (110.40 to 358.80 kg ha-1), available phosphorus content (9.35 to 29.92 kg ha-1), available potassium (117.60 to 362.88 kg ha-1). Likely, the physico-chemical properties of the soil and available nutrients of Koppal district samples ranged from, pH (6.30-8.50), electrical conductivity (0.12-0.85 dS m-1), organic carbon (0.12-0.91%), available nitrogen (188.60 to 552.10 kg ha-1), available phosphorus content (8.66 to 35.07 kg ha-1), and available potassium (117.60 to 406.54 kg ha-1). Forty Azospirillum isolates were obtained by adopting enrichment culture technique. All the isolates formed subsurface pellicles in NFBTB medium including the reference strain. Pellicle was formed 1-2 mm from the surface of semisolid NFBTB medium. All the Azospirillum strains turned olive green colour of Bomothymol Blue (BTB) to brilliant blue.


Keywords: Azospirillum spp, Physico-chemical properties and NFBTB
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How to cite this article:

Vijayalakshmi, N.R. and Mahadeva Swamy. 2019. Characterization of Rhizosphere Soils of Foxtail Millet and Isolation of Azospirillum Strains.Int.J.Curr.Microbiol.App.Sci. 8(3): 1688-1696. doi: https://doi.org/10.20546/ijcmas.2019.803.196