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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 1612-1618
DOI: https://doi.org/10.20546/ijcmas.2019.803.187


The First Determination of the Plasmid-Mediated Quinolone Resistance Determinants qnrA and qnrB from the Sudan
I.A. Malik1* and K.M. Elhag2
1Liverpool Tropical school of Medicine.UK, PhD Medical Microbiology (Liverpool University.UK), Ahfad University for Women-Sudan
2Ahfad University for Women, Soba University Hospital-Sudan
*Corresponding author
Abstract:

The aim of this study was to determine the prevalence of plasmid-mediated quinolone resistance qnr genes (A, B and S) among a collection of enterobacterial clinical pathogens resistant to ciprofloxacin and was ESBL producers from Sudan. Seventy-two isolates that were ciprofloxacin resistant and ESBL producer were screened by PCR for qnr genes (A, B and S). qnrA positive isolates were tested by PCR for class-1 integrons as well as resistance transfer by conjugation with sodium azide-resistant Escherichia coli J53. DNA-relatedness was tested by PFGE. Qnr genes were detected among 13% of the test isolates, seven isolates were qnrB1 positive, included an E.coli and six Klebsiella pneumoniae isolates. qnrA was detected among two Enterobacter cloacae isolates. Genomic analysis by PFGE on the two qnrA positive isolates revealed two closely related organisms. Plasmid transfer of quinolone resistance was achieved on the two qnrA positive isolates, both plasmids showed part of the 3′-CS with the aminoglycoside-3′-adenyltransferases aadA2 and aacA4 genes as well as part of the Intl1 gene which was In6-like class-1 integrons. This study demonstrated high prevalence of qnrB (10%) among the test isolates compared to qnrA (3%) and non of the qnr S. This is the first report on plasmid-mediated ciprofloxacin resistance genes (qnrgenes) from Sudan.


Keywords: qnr, plasmid, Genes, Sudan, Ciprofloxacin
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How to cite this article:

Malik, I.A. and Elhag, K.M. 2019. The First Determination of the Plasmid-Mediated Quinolone Resistance Determinants qnrA and qnrB from the Sudan.Int.J.Curr.Microbiol.App.Sci. 8(3): 1612-1618. doi: https://doi.org/10.20546/ijcmas.2019.803.187