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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 1436-1448
DOI: https://doi.org/10.20546/ijcmas.2019.803.168


In vitro Inductions of Gamma Ray Mutation for Fusarium Wilt Resistance and Characterization of Mutants in Regionally Grown Banana Cultivars
Rashmi Hegde1*, .G Prabhuling5, Kulpathi Hipparagi2, A.G. Babu5, C.P Mansur3 and D. Satish4
1Department of Biotechnology and Crop Improvement, University of Horticultural Sciences, Bagalkot, India
2Department of Fruit Science, University of Horticultural Sciences, Bagalkot, india
3Department of Agronomy, University of Agricultural Sciences, Dharwad, India
4Department of Biotechnology and Crop Improvement, Arbhavi, University of Agricultural Sciences, Dharwad, India
5Centre for Horticulture Biotechnology, Directorate of Research, University of Horticultural Sciences, Bagalkot, India
*Corresponding author
Abstract:

Regionally grown banana cultivars are severely infected by several pathogens one such Fusarium oxysoporum f. sp. Cubense which causes significant crop losses in the fields. In this study fusarium wilt screening was carried out on gamma ray induced mutants and molecular characterization was done to assess the acquired resistance to Fusarium oxysoporum f. sp. cubense. From the screening we observed that gamma ray derived mutants are more sensitive in 25, 30 and 35 Gy. Whereas, several mutants obtained from 40 and 45 were resistant as comparable with untreated control. Similar analysis with molecular markers also revealed comparable results in screening. SSR primers showed resistance specific bands and were used for the testing variation among mutants, after confirming its reproducibility. Putative resistant, moderate resistant and susceptible mutants along with untreated control banana plants when amplified with Ma 3/2 and Ma 15 in Nanjanagudu Rasabale showed the presence of a major band at 150 bp and 500 bp and in Ma 1/17 and STMS 7 in Ney Poovan showed the presence 500 bp and 250 bp as major bands, this evidenced the occurrence of random mutations in genome.


Keywords: Mutation, Screening, Fusarium wilt, characterization, SSR, disease resistance
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How to cite this article:

Rashmi Hegde, .G Prabhuling, Kulpathi Hipparagi, A.G. Babu, C.P Mansur and Satish, D. 2019. In vitro Inductions of Gamma Ray Mutation for Fusarium Wilt Resistance and Characterization of Mutants in Regionally Grown Banana Cultivars.Int.J.Curr.Microbiol.App.Sci. 8(3): 1436-1448. doi: https://doi.org/10.20546/ijcmas.2019.803.168