Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 628-634
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.069


Species Distribution, Biofilm Formation and Antifungal Susceptibility of Candida Isolates in Blood Samples of NICU Patients at Tertiary Care Centre, Amritsar, India
Rajesh Bansal*, Loveena Oberoi, Kanwardeep Singh and Pushpa Devi
Department of Microbiology, Government Medical College, Amritsar, India
*Corresponding author
Abstract:

To study the distribution, biofilm formation and antifungal susceptibility pattern of Candida species isolated from neonatal candidemia patients. Candida infections are a serious problem in neonatal intensive care units leads to increasing morbidity and mortality. A prospective observational study was conducted from September 2014 to June 2016. A total of 313 blood samples received in Microbiology department from NICU patients with suspected Candida infection were collected. Samples were processed by Gram staining, KOH mount & culture on SDA and BHI agar. Isolated yeasts were identified and speciated by germ tube test, chlamydospores formation on corn meal agar, color production on CHROM agar, sugar fermentation test and sugar assimilation test. Antifungal susceptibility testing of isolates was performed as per CLSI guidelines. Biofilm production was tested by Tube method and Tissue culture plate method. The data was statistically analysed using the statistical package for Social science (SPSS)/21.0.  A total of 60 (19.17%) samples out 313 blood samples were positive for candida infections. Most common fungal isolate was Candida tropicalis (45%) followed by Candida albicans (25%), C. parapsilosis (11.7%), C. glabrata (11.7%) and C. krusei (6.7%.). Maximum resistance was observed to Fluconazole & Ketoconazole. Antifungal resistant was found to be more in biofilm producers. This Study highlights the emergence of Non albicans Candida as major isolates and increasing antifungal resistance.


Keywords: NICU (neonatal intensive care unit); Antifungal susceptibility testing; biofilm.
Download this article as Download

How to cite this article:

Rajesh Bansal, Loveena Oberoi, Kanwardeep Singh and Pushpa Devi. 2016. Species Distribution, Biofilm Formation and Antifungal Susceptibility of Candida Isolates in Blood Samples of NICU Patients at Tertiary Care Centre, Amritsar, India.Int.J.Curr.Microbiol.App.Sci. 5(12): 628-634. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.069