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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 1246-1252
DOI: https://doi.org/10.20546/ijcmas.2019.803.147


Validation of Microsatellite Markers for Parentage Verification in Indian HF Cattle
A.S. Khade1*, M.P. Sawane1, V.D. Pawar1, B.K. Mauryaand R.R. Nair2
1Department of Animal Genetics and Breeding, Bombay Veterinary College, Mumbai, Maharashtra, India
2Centre for Analysis and Learning in Livestock and Food, NDDB, Anand, Gujarat, India
*Corresponding author
Abstract:

The study was undertaken with an objective to develop and validate a panel containing maximum number of microsatellite markers that can be amplified in a single PCR reaction for precise parentage verification in Indian HF cattle population. The study was based on a total of 210 HF cattle (100 calf, 100 dam and 10 sires). Genomic DNA was extracted from blood and semen samples. A panel of 12 microsatellite markers (BM1824, BM2113, INRA023, SPS115, TGLA122, TGLA126, TGLA227, ETH10, ETH225, BM1818, ETH3, TGLA53) was amplified in a single multiplex reaction and analyzed by capillary electrophoresis on an automated DNA sequencer. The observed heterozygosity (Ho) of 12 markers ranged from 0.607 (TGLA53) to 0.904 (BM2113) while the expected heterozygosity (He) ranged from 0.581 (ETH225) to 0.873 (INRA23). Eleven out of 12 microsatellite loci revealed relatively high polymorphic information content (>0.6). The results suggest that multiplex microsatellite panel can be effectively used to verify the parentage as well as to assign the putative sire to daughters under progeny testing.


Keywords: HF, Microsatellite, Multiplex Polymerase Chain Reaction, Fragment Analysis, Parentage Verification
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How to cite this article:

Khade, A.S., M.P. Sawane, V.D. Pawar, B.K. Maurya and Nair, R.R. 2019. Validation of Microsatellite Markers for Parentage Verification in Indian HF Cattle.Int.J.Curr.Microbiol.App.Sci. 8(3): 1246-1252. doi: https://doi.org/10.20546/ijcmas.2019.803.147