National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Indole acetic acid (IAA) is one of the most important phytohormones enhances the root structure and plant growth. The present study provides the isolation and characterization of IAA producing bacteria from rhizosphere soil and their effect on seed germination. Out of 30 isolates, eight (viz. Aux 4, Aux 9, Aux14, Aux16, Aux 19, Aux 20, Aux 21 and Aux 25) were able to produce IAA which was confirmed by Salkowski reagent test. Isolates were characterized on the basis of visual observations, gram staining and biochemical tests such as oxidase, catalase, indole, TSI, methyl red, Voges-Proskauer, citrate utilization, urease and starch hydrolysis test. The IAA producing bacteria are rod and round shaped gram positive and gram negative bacteria. In biochemical tests, both positive and negative responses were observed. Spectrometric analysis of IAA was done after 24, 48 and 72 hours of culture which showed that the isolated bacteria produced maximum concentration of IAA after 72 hours of incubation period at 37°C. The concentration was measured using a standard IAA curve and the maximum concentration was obtained by Aux 25. The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control. Further study is necessary for molecular characterization of isolated bacteria.
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