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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 1237-1245
DOI: https://doi.org/10.20546/ijcmas.2019.803.146


Isolation and Characterization of Indole Acetic Acid Producing Bacteria from Rhizosphere Soil and their Effect on Seed Germination
Sharnali Das, Tauhidur Rahman Nurunnabi, Rehana Parveen, Amatun Nur Mou, Md. Emdadul Islam, Kazi Mohammad Didarul Islam and S.M. Mahbubur Rahman*
Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna-9208, Bangladesh
*Corresponding author
Abstract:

Indole acetic acid (IAA) is one of the most important phytohormones enhances the root structure and plant growth. The present study provides the isolation and characterization of IAA producing bacteria from rhizosphere soil and their effect on seed germination. Out of 30 isolates, eight (viz. Aux 4, Aux 9, Aux14, Aux16, Aux 19, Aux 20, Aux 21 and Aux 25) were able to produce IAA which was confirmed by Salkowski reagent test. Isolates were characterized on the basis of visual observations, gram staining and biochemical tests such as oxidase, catalase, indole, TSI, methyl red, Voges-Proskauer, citrate utilization, urease and starch hydrolysis test. The IAA producing bacteria are rod and round shaped gram positive and gram negative bacteria. In biochemical tests, both positive and negative responses were observed. Spectrometric analysis of IAA was done after 24, 48 and 72 hours of culture which showed that the isolated bacteria produced maximum concentration of IAA after 72 hours of incubation period at 37°C. The concentration was measured using a standard IAA curve and the maximum concentration was obtained by Aux 25. The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control. Further study is necessary for molecular characterization of isolated bacteria.


Keywords: Indole acetic acid (IAA), Bacterial isolates, Inoculums, Biochemical characteristics, Rhizosphere soil and Germination
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How to cite this article:

Sharnali Das, Tauhidur Rahman Nurunnabi, Rehana Parveen, Amatun Nur Mou, Md. Emdadul Islam, Kazi Mohammad Didarul Islam and Mahbubur Rahman, S.M. 2019. Isolation and Characterization of Indole Acetic Acid Producing Bacteria from Rhizosphere Soil and their Effect on Seed Germination.Int.J.Curr.Microbiol.App.Sci. 8(3): 1237-1245. doi: https://doi.org/10.20546/ijcmas.2019.803.146