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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 614-619
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.067


Qualitative Assessment of Allergen Skin Test and In vitro Allergen Specific Immunoglobulin E (IgE) Measurement as a Method of Detection of Allergic Symptoms
K. Goswami1, I. Mazumdar2*, S. Mookherjee3 and P. Pal4
1Department of Biochemistry, Lincoln University College, Kuala Lumpur, Malaysia
2Department of Biochemistry, KPC Medical College, Kolkata, India
3Biochemist, North City Diagnostic Centre, Kolkata, India
4Department of Pharmacology, IIMSR & B C Roy Hospital, Haldia, India
*Corresponding author
Abstract:

The increase in air pollutants affects both mortality and morbidity of the respiratory system. People are exposed to various indoor allergens like dust mite, pests and pet shedding, manifesting as Asthma. The diagnosis of allergy is either by allergen specific skin test (SPT) or in vitro specific allergen mediated Immunoglobulin E (IgE) measurement. Allergen specific IgE is indicated for evaluating allergy as a more authentic and specific diagnostic tool. But this has not yet been explored much till date. The present study assessed qualitatively the importance of both SPT and allergen specific IgE measurement for diagnosis of allergy. This study was conducted upon bronchial asthma patients. Allergy SPTs were carried out using commercially available antigens. The concentrations of allergen specific IgE antibodies to allergens with most positive skin test reaction were determined using the rapid, solid phase enzyme linked immunosorbent assay. Trends of reaction patterns remained similar in both. Regression analysis studies revealed positive correlation between the tests following some allergens. But corresponding correlation in all forms of allergens could not be established. Therefore characterization and standardization for the commercial allergen and specific IgE assay kit were proposed. In conclusion, specific IgE to aeroallergens seemed a more specific tool for detecting allergen specificity, though SPT seemed to be more sensitive.


Keywords: House Dust Mite, Asthma,Skin Prick Test, Immunoglobulin E, Environmental Allergens.
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How to cite this article:

Goswami, K., I. Mazumdar, S. Mookherjee and Pal, P. 2016. Qualitative Assessment of Allergen Skin Test and In vitro Allergen Specific Immunoglobulin E (IgE) Measurement as a Method of Detection of Allergic SymptomsInt.J.Curr.Microbiol.App.Sci. 5(12): 614-619. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.067