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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 8, Issue:3, March, 2019

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 861-865
DOI: https://doi.org/10.20546/ijcmas.2019.803.103


Isolation and Characterization of Photosynthetic Bacteria from Municipal Waste
Malik Sajad Ahmad1*, M.Y. Zargar2, S.A. Mir4, N.A. Bhat3, Z.A. Baba1, Rehana Habib Kant5, Zaffar M. Dar1, Imtiyaz Jahangir Khan1 and Jigmet Yangchan6
1Division of Basic Sciences and Humanities
2Directorate of Research
3Division of Plant Pathology
4Division of Agri. Statistics
5Division of Agronomy, Faculty of Agriculture
6HMAARI Leh, SKUAST-K, Shalimar, Srinagar, 190025
*Corresponding author
Abstract:

One of the photosynthetic bacterial isolate L6 grown at 10 oC confirmed as Rhodoferax sp. and six isolates showed growth at 30 °C were isolated from municipal wastes in Kashmir and Ladakh valleys. The cells were Gram-negative, pigmented, non-spore-forming, mostly oxidase positive except K2 isolate and nitrate reduction negative except G17 isolate. All isolates showed growth in aerobic-light conditions except K2 and anaerobic-light except L6. All isolates utilized acetate, malate and succinate as carbon source while as benzoate and mannitol were not used except G17. However mannitol was used as carbon source by L6 also. Isolate K2 had shown well defined growth in sulphide medium and was regarded as sulphur bacterium while as isolates G17, L6, L27, K5, S5 and S7 were unable to utilize sulphide and thiosulphate as electron donor and considered as non sulphur bacteria. The morpho-biochemical test further confirmed K2 isolate as Chlorobium sp., S5 and L27 as Rhodospirillum sp., S7 as Rhodobacter sp., G17 as Rhodopseudomonas sp., K5 as Rhodomicrobium sp. Isolates G17, L6, L27, S5 and S7 were observed the most efficient on the basis of versatility in carbon utilization character. Isolate L6 was regarded as the psychrophillic/psychrotolerant photosynthetic purple non sulphur bacterium.


Keywords: Anaerobic-light, benzoate, Chlorobium sp., Gram- negative, Rhodoferax sp., Rhodopseudomonas

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How to cite this article:

Malik Sajad Ahmad, M.Y. Zargar, S.A. Mir, N.A. Bhat, Z.A. Baba, Rehana Habib Kant, Zaffar M. Dar, Imtiyaz Jahangir Khan and Jigmet Yangchan. 2019. Isolation and Characterization of Photosynthetic bacteria from Municipal Waste.Int.J.Curr.Microbiol.App.Sci. 8(3): 861-865. doi: https://doi.org/10.20546/ijcmas.2019.803.103
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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