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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 583-593
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.063


Participation of an Inactive and Active Src-kinase in Formation of a Cytoskeleton and Melanogenesis in Hep2 Cells
Tatiana Yavisheva* and Sergey Shcherbakov
Joint Stock Company “R-PHARM”, Moscow, Russian Federation
*Corresponding author
Abstract:

The present study investigated the participation of an inactive and active Src-kinase in the functions of cytoskeleton and pigment formation inHep2 cells. For increase of the inactive portion of the Src-kinase and decrease of its active part we entered specific Src-kinase family inhibitor CGP 77675 into cell population. For the decrease of the inactive portion of Src-kinase, the tumor cells were affected by ultraviolet (UV) radiation within 5, 30 and 60 minutes. We studied the dynamics of the quantity of cells with processes and also the cells with tyrosinase activity. It appeared that only the inactive Src-kinase participates in cytoskeleton formation and in tyrosinase activization. Strengthening of melanogenesis during long UV-radiation within 30 and 60 minutes reduced the portion of the inactive Src-kinase due to the intensification of tyrosinase synthesis. The last in turn began to compete with a cytoskeleton for the inactive Src-kinase that reduced a differentiation of cells and sharply worsened a phenotype of tumor cells.


Keywords: Active and inactive Src-kinase, cytoskeleton formation, melanogenesis.
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How to cite this article:

Tatiana Yavisheva and Sergey Shcherbakov. 2016. Participation of an Inactive and Active Src-kinase in Formation of a Cytoskeleton and Melanogenesis in Hep2 Cells.Int.J.Curr.Microbiol.App.Sci. 5(12): 583-593. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.063