Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 822-835
DOI: https://doi.org/10.20546/ijcmas.2019.803.099


Isolation, Cloning and Sequencing of phlACBD Gene Cluster Encoding Antibiotic and Phloroglucinol Derivative 2, 4-diacetylphloroglucinol (2, 4-DAPG) from Pseudomonas fluorescens
T.M. Ningaraju1*, H.V. Chaithra1 and Anitha Peter2
1Department of plant Biotechnology, College of Sericulture, Chintamani, UAS, Bangalore, India
2Department of plant Biotechnology, GKVK, UAS, Bangalore, India
*Corresponding author
Abstract:

Pseudomonas represents as one of the most abundant class of bacterial genus in many antagonistic root-associated communities. Pseudomonas spp. residing in the rhizosphere has the ability to produce antimicrobial metabolites including phloroglucinol derivatives 2,4-DAPG, which is a major class of secondary metabolite protecting plants against different types of phytopathogens. The present study was conducted to isolate 2,4-DAPG producing Pseudomonas fluorescens isolates from Rhizosphere soil samples of different crops. Among a total of 158 isolates screened for the production of 2,4-DAPG through PCR based approach, seven isolates were found to be positive for DAPG. Further, the antibacterial activities of these Pseudomonas isolates were evaluated in vitro against Xanthomonas axonopodis pv punicae and it was found that the isolate AFPF19 exhibited highest antagonistic activity against phytopathogen. AFPF19 isolate was used to clone DAPG synthesizing gene cluster phlACBD into cloning vector pTZ257R/T and confirmed by sequencing.


Keywords: Pseudomonas fluorescens, Antagonistic, 2, 4-diacetylphloroglucinol, Cloning, Rhizosphere
Download this article as Download

How to cite this article:

Ningaraju, T.M., H.V. Chaithra, and Anitha Peter. 2019. Isolation, Cloning and Sequencing of phlACBD Gene Cluster Encoding Antibiotic and Phloroglucinol Derivative 2, 4-diacetylphloroglucinol (2, 4-DAPG) from Pseudomonas fluorescens.Int.J.Curr.Microbiol.App.Sci. 8(3): 822-835. doi: https://doi.org/10.20546/ijcmas.2019.803.099