Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 766-774
DOI: https://doi.org/10.20546/ijcmas.2019.803.094


Phenotypic and Symbiotic Diversity of Rhizobia Isolated from Root Nodules of Soybean [Glycine max (L.) Merrill] in Côte d’Ivoire
Amani Kouadio1, Konate Ibrahim1*, Mako François De Paul N’gbesso2, Attien Yao Paul1, Lassina Fondio2, Abdelkarim Filali-Maltouf3 and Tidou Abiba Sanogo1
1UFR Agro-forestry and Environment, Department of Biochemistry and Microbiology, Laboratory of Agrovalorization, Jean Lorougnon Guede University, B.P: 150 Daloa, Côte d’Ivoire
2National Center for Agronomic Research (CNRA), 01 BP 633 Bouaké 01, Côte d’Ivoire
3Faculty of Sciences, Laboratory of Microbiology and Molecular Biology, University Mohammed V-Agdal, Rabat, Morocco
*Corresponding author
Abstract:

Soybean [Glycine max (L.) Merrill] is a legume known for nitrogen fixation interacting with efficient Bradyrhizobium strains. In Côte d'Ivoire, the Bradyrhizobium inoculums used in soybean production is a foreign strain, which requires the search for indigenous strains adapted to Ivorian soils. The present study aimed to isolate and select rhizobial bacteria from soybeans grown on soils sampled from six localities in the central-western region of Côte d'Ivoire. Thirty bacteria were obtained from the nodules of plants grown on soils of two localities that had previously received soybean cultivation (Gonate North and South). These isolates were coded as RSC and identified as being able to genus Bradyrhizobium (slow growth) and Sinorhizobium (intermediate growth) depending on cultural and morphological characteristics. Except for RSC 327 and RSC 330, all isolates were authenticated and were able to nodulate the host plant in controlled culture. The isolates RSC 309, RSC 310, RSC 312, RSC 323, RSC 324, RSC 325 and RSC 326 were efficient and significantly (P <5%) increased the number and weight of the nodules, the height and plant biomass. Moreover, these same isolates also showed a high tolerance to salt (NaCl) added in YEM media at a concentration ranging from 4 to 5%. Based on the symbiotic and physiological characteristics, isolates RSC 309, RSC 310, RSC 312, RSC 323, RSC 324 and RSC 325 could be recommended as the native soybean inoculums under soil and climatic conditions of Côte d’Ivoire.


Keywords: Rhizobia, Soybean, Native strains, Selection, Côte d’Ivoire
Download this article as Download

How to cite this article:

Amani Kouadio, Konate Ibrahim, Mako François De Paul N’gbesso, Attien Yao Paul, Lassina Fondio, Abdelkarim Filali-Maltouf and Tidou Abiba Sanogo. 2019. Phenotypic and Symbiotic Diversity of Rhizobia Isolated from Root Nodules of Soybean [Glycine max (L.) Merrill] in Côte d’Ivoire.Int.J.Curr.Microbiol.App.Sci. 8(3): 766-774. doi: https://doi.org/10.20546/ijcmas.2019.803.094