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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 733-744
DOI: https://doi.org/10.20546/ijcmas.2019.803.090


Bioremediation of Crude Oil Polluted Terrestrial Soil using Aspergillus clavatus and Pichia spp.
Salome Ibietela Douglas1 and Barisi Samuel Penu2*
1Department of Microbiology, Faculty of Science, Rivers State University, PMB 5058, Nkpolu-Oroworukwo, Port Harcourt, Rivers State, Nigeria
2Department of Science Laboratory Technology, School of Applied Sciences, Kenule Beeson Saro-Wiwa Polytechnic, P. M. B. 20, Bori, Rivers State, Nigeria

*Corresponding author
Abstract:

In Nigeria, crude oil pollution challenge of the terrestrial environment is very prevalent. Soil fungi play very important role in the degradation of organic materials, as well as agents of the biogeochemical cycles. Fungi have an advantage over bacteria due to their production of hyphae that can penetrate contaminated soil. The aim of this study was to evaluate the bioremediation potential of two fungal species: Aspergillus clavatus and Pichia spp. Crude oil contaminated soil samples were collected from Numuu Mitee, Kegbara-Dere community in Gokana Local Government Area of Rivers State; Nigeria. There were four experimental setups for the bioremediation study; Soil without organisms served as control(A), soil with Aspergillus clavatus alone (B), soil sample with Pichia spp. alone (C), while soil with Aspergillus clavatus and Pichia spp combined (D). Standard microbiological methods were used to analyze total heterotrophic and hydrocarbon utilizing fungi. The following physicochemical parameters; pH, nitrate, phosphate, sulphate, and total hydrocarbon content (THC) were analysed for baseline and monitored every 7days for 28days. Molecular identification of the organisms was also carried out using 16S rRNA amplification. The results of the baseline were as follows; pH 6.9, nitrate 52mg/kg, phosphorus 149mg/kg, THC 8,006.58mg/kg, total heterotrophic fungi 3.8 x 104cfu/g and hydrocarbon utilizing fungi 2.3 x 103cfu/g. The results showed that the physicochemical parameters decreased significantly during the study period. The results of bioremediation indicates that the total hydrocarbon content of the soil in day 1 was 8006.58mg/kg but reduced in day 28 to 6799.74mg/kg for setup A, 3309.21mg/kg for B, 2835.53mg/kg for C and 1572.37mg/kg for D. The percentage THC loss was in this order: D>C>B>A, 80.36% > 64.59% > 58.67% > 15.07% respectively. This study reveals that using the Pichia species alone produced 64% THC loss while Aspergillus clavatus alone produced 58.6% loss. Combined potential of Aspergillus clavatus and Pichia spp. produced 80% reduction in 28days. This makes the consortium a more efficient option in bioremediation of crude oil contaminanted terrestrial soil.


Keywords: Bioremediation, Crude oil, Aspergillus clavatus, Pichia spp, Terrestrial soil
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How to cite this article:

Salome Ibietela Douglas and Barisi Samuel-Penu. 2019. Bioremediation of Crude Oil Polluted Terrestrial Soil using Aspergillus clavatus and Pichia spp.Int.J.Curr.Microbiol.App.Sci. 8(3): 733-744. doi: https://doi.org/10.20546/ijcmas.2019.803.090