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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 596-608
DOI: https://doi.org/10.20546/ijcmas.2019.803.073


Expression Profiling of Resistance Gene Analogs from French Bean (Phaseolus vulgaris L.) for the Manifestation of Moong Bean Yellow Mosaic Virus
B. Divya*, B. Fakrudin and C. Ashwat
College of Horticulture, Bengaluru, University of Horticulutural Sciences, Bagalkot Indian Institute of Horticultural Sciences, Hesarghatta, Bengaluru, India
*Corresponding author
Abstract:

The popular and susceptible french bean genotype Arka Anoop was used for expression profiling of RGAs for the manifestation of MYMV. Leaf tissue was collected from both disease free and artificially disease challenged plants at 7th, 15th and 30th DAI and syntesized cDNA. The expression level of selected 10 RGA genes of french bean was measured in both disease free and artificially inoculated leaf tissues separately at 7th, 15th and 30th DAI. 10 RGA genes were tested under MYMV infested condition in french bean. All 10 selected RGA genes were expressed in leaf tissues at different days after inoculation with MYMV. COHFBRGA-2, 6, 8 and 10 genes of french bean were up-regulated in leaf of susceptible genotypes at disease inoculation condition at all the intervals. COHFBRGA-3, 4, 5 and 7 was down- regulated among all the intervals of disease development compared to control. Whereas, COHFBRGA-9 expressed only at 30 DAI.


Keywords: French bean, RGAs, Arka Anoop, MYMV and Expression analysis
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How to cite this article:

Divya, B., B. Fakrudin and Ashwat, C. 2019. Expression Profiling of Resistance Gene Analogs from French Bean (Phaseolus vulgaris L.) for the Manifestation of Moong Bean Yellow Mosaic Virus.Int.J.Curr.Microbiol.App.Sci. 8(3): 596-608. doi: https://doi.org/10.20546/ijcmas.2019.803.073