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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(3): 82-82
DOI: https://doi.org/10.20546/ijcmas.2019.803.012


Regeneration of Indica Rice (Oryza sativa L.) from Calli Derived through Immature Embryos
Bhakti Prakash Mallik1*, Barsita Swain2, Sangeeta Kujur3 and Jagadish Jena 
1MITS School of Biotechnology, India
2Orissa University of Agriculture and Technology, Choudhury Radhagobinda Routray, Utkal University, India
3IGKV, Raipur, India
*Corresponding author
Abstract:

A new and rapid method for optimum callus production was established in 6 rice (Oryza sativa) varieties. Immature seeds were used as a starting material for callus induction using different concentrations of BAP, NAA, and Kinetin. Higher percentage of callus induction was obtained in MS + 0.5mg/l BAP + 1.0mg/l NAA + 3% sucrose. Healthy calli emerged which were compact in texture and globular, light yellowish in colour and also it was observed that the calli of the variety, INH1001 shows early and rapid growth in callus size within 14 days of incubation as compared to other varieties used in this study. For calli regeneration, induced calli of higher efficiency INH1001, along with other five varieties were treated with MS media supplemented with various concentrations of BAP (0.5-2.0mg/l), NAA (0.1-1.0 mg/l) and 5mg/l proline. The shoot regeneration was found maximum in MS media supplemented with 2.0 mg/l BAP+ 0.1 mg/l NAA + 5mg/l proline within 1 week of culture; it was observed that the calli of INH1001 variety shows early regeneration within 7 days of incubation as compared to other varieties. However, other calli responded to late shoot regeneration.


Keywords: Oryza sativa, Regeneration frequency, BAP, Kinetin, NAA, Proline
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How to cite this article:

Bhakti Prakash Mallik, Barsita swain, Sangeeta Kujur and Jagadish Jena. 2019. Regeneration of Indica Rice (Oryza sativa L.) from Calli Derived through Immature Embryos.Int.J.Curr.Microbiol.App.Sci. 8(3): 82-82. doi: https://doi.org/10.20546/ijcmas.2019.803.012