National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study mainly aimed to study the prevalence of antibiotics resistance among E. coli isolated from community acquired urinary tract infections (UTIs) and to determine the prevalence of AcrA/B and tolC efflux genes among the isolated E. coli. The study was a cross-sectional study that included 100 E. coli strains isolated from patients with community acquired UTIs. Antibiotics susceptibility to E. coli was determined by disc diffusion method. Efflux genes AcrA/B and tolC were determined by polymerase chain reaction (PCR). The prevalence of efflux genes in the isolated E. coli were 71% for each of acrB and tolC genes and 55% for acA gene. The antibiotics resistance of E. coli was significantly associated with the presence of acrA/B and TolC genes among different antibiotics such as amikacin, ampicillin and cefuroxime (P=0.0001). While, resistance to nalidixic acid, trimethoprim-sulfamethoxazole, levofloxacin and gentamicin had non-significant association with the presence of the efflux genes. The studied efflux genes show that the genes were significantly associated with each other, P=0.0001. AcroA gene was present in combination with acroB and tolC in 51 isolates and acrB with tolC in 61 isolates only 16 isolates had one gene either acrB or tolC. The results of the present study suggest increase in the prevalence of antibiotics resistance in E. coli associated with community acquired urinary tract infections. This resistance may be attributed to irrational use of the antibiotics and the presence of efflux genes that result in antibiotics resistance. The acrA/B and tolC genes had high prevalence in E. coli.
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