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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(11): 3647-3655
DOI: https://doi.org/10.20546/ijcmas.2018.711.419


Study of Efflux Genes in E. coli Isolated from Community Acquired Urinary Tract Infections
Mohamed Anies Rizk1* and Rasha Mahmoud2
1Department of Clinical Pathology, 2Department of Internal Medicine, Mansoura Faculty of Medicine, Egypt
*Corresponding author
Abstract:

The present study mainly aimed to study the prevalence of antibiotics resistance among E. coli isolated from community acquired urinary tract infections (UTIs) and to determine the prevalence of AcrA/B and tolC efflux genes among the isolated E. coli. The study was a cross-sectional study that included 100 E. coli strains isolated from patients with community acquired UTIs. Antibiotics susceptibility to E. coli was determined by disc diffusion method. Efflux genes AcrA/B and tolC were determined by polymerase chain reaction (PCR). The prevalence of efflux genes in the isolated E. coli were 71% for each of acrB and tolC genes and 55% for acA gene. The antibiotics resistance of E. coli was significantly associated with the presence of acrA/B and TolC genes among different antibiotics such as amikacin, ampicillin and cefuroxime (P=0.0001). While, resistance to nalidixic acid, trimethoprim-sulfamethoxazole, levofloxacin and gentamicin had non-significant association with the presence of the efflux genes. The studied efflux genes show that the genes were significantly associated with each other, P=0.0001. AcroA gene was present in combination with acroB and tolC in 51 isolates and acrB with tolC in 61 isolates only 16 isolates had one gene either acrB or tolC. The results of the present study suggest increase in the prevalence of antibiotics resistance in E. coli associated with community acquired urinary tract infections. This resistance may be attributed to irrational use of the antibiotics and the presence of efflux genes that result in antibiotics resistance. The acrA/B and tolC genes had high prevalence in E. coli.


Keywords: E. coli, UTIs, Efflux genes
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How to cite this article:

Mohamed Anies Rizk and Rasha Mahmoud. 2018. Study of Efflux Genes in E. coli Isolated from Community Acquired Urinary Tract Infections.Int.J.Curr.Microbiol.App.Sci. 7(11): 3647-3655. doi: https://doi.org/10.20546/ijcmas.2018.711.419