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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 3379-3383
DOI: https://doi.org/10.20546/ijcmas.2019.802.393


Validation of SCAR Marker Linked to Genic Male Sterility in Marigold: As a Forward Step towards Marker Assisted Breeding Programme
K.M. Asha1*, Anuradha Sane1, Tejaswini1, D.C. Lakshaman Reddy1, Sateesha R. Patil2, Sarvamangala S. Cholin3, Mahantesha B.N. Naika2 and Raghavendra Gunnaiah3
1IIHR Hessaraghatta; Division of ornamental crops, IIHR, Hessaraghatta, Bengaluru- 560089, India
2College of Horticulture, Arabhavi, India
3College of Horticulture, Bagalkot, India
*Corresponding author
Abstract:

The identification of marker tightly linked to male sterility will greatly facilitate for marker assisted selection (MAS) breeding through accurate selection of parental lines in hybrid production. In the present study, to assess the efficiency of previously reported SCAR 4 marker for marker assisted selection was validated by screening the marker in a total of 226 F2 mapping population derived from a cross between male sterile (IIHR10521AB) and a male fertile pure line (IIHRMY7) maintained at IIHR along with bulk segregant analysis. The results showed that the marker segregated in the F2 population showing that it is linked to sterility locus. The marker was also validated by screening 12 different apetalloid male sterile lines maintained at IIHR, the results of amplification gave clear and similar band size amplicons present in parents in all the apetalloid sterile lines confirming that it is linked to male sterility and hence this study is significantly useful and can offer a powerful tool for the efficient selection in MAS breeding programmes in marigold.


Keywords: Marigold, Genic Male sterility, SCAR marker, Linked marker, MAS, Validation, Apetalloid sterile lines
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How to cite this article:

Asha, K.M., Anuradha Sane, Tejaswini, D.C. Lakshaman Reddy, Sateesha R. Patil, Sarvamangala S. Cholin, Mahantesha B.N. Naika and Raghavendra Gunnaiah. 2019. Validation of SCAR Marker Linked to Genic Male Sterility in Marigold: As a Forward Step towards Marker Assisted Breeding Programme.Int.J.Curr.Microbiol.App.Sci. 8(2): 3379-3383. doi: https://doi.org/10.20546/ijcmas.2019.802.393