Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2019) [Effective from January 1, 2019]For more details click here

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 3282-3290
DOI: https://doi.org/10.20546/ijcmas.2019.802.383


Molecular and Computational Studies of Trichoderma Isolates
Ved Ratan1, Mukesh Srivastava2, Supriya Dixit3*, D.K. Srivastava4, Shubha Trivedi3 and Yatindra Kumar Srivastava1
1Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur (208002), India
2Rani Laxmi Bai Central Agricultural University, Jhansi (U.P.) 284003, India
3Biocontrol Lab, Department of Plant Pathology, Chandra Shekhar Azad University of Agriculture & Technology, Kanpur 208002, India
4Council of Science and Technology, Lucknow, India
*Corresponding author
Abstract:

Most isolates of the genus Trichoderma were found to act as mycoparasites of many economically important aerial and soil-borne plant pathogens. Trichoderma has gained importance as a substitute for chemical pesticides and hence an attempt was intended to corroborate the positive relatedness of molecular and morphological characters. Fungal strains of Trichoderma BSAs were isolated collected from four different districts of Uttar Pradesh, India. The universal primers were used for amplification of 5.8SrRNA gene fragment and the strain was characterized by using 5.8SrRNA gene sequence with the help of ITS marker. It is proposed that the identified isolates assigned as the species of the genus Trichoderma based on TrichoKey analysis together with the 5.8SrRNA gene sequence search in Ribosomal Database Project, small subunit rRNA and large subunit rRNA databases. Thus the molecular markers can be employed to identify a superior strain of Trichoderma for its commercial exploitation.


Keywords: 5.8S ribosomal RNA gene, Trichoderma, ITS, TrichoKey, TrichoBLAST
Download this article as Download

How to cite this article:

Ved Ratan, Mukesh Srivastava, Supriya Dixit, D.K. Srivastava, Shubha Trivedi and Yatindra Kumar Srivastava. 2019. Molecular and Computational Studies of Trichoderma Isolates.Int.J.Curr.Microbiol.App.Sci. 8(2): 3282-3290. doi: https://doi.org/10.20546/ijcmas.2019.802.383