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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 3203-3208
DOI: https://doi.org/10.20546/ijcmas.2019.802.374


Sap Transmission and Detection of Sunflower Necrosis virus in Sunflower
Ravi Kumar*, B. Jagadeesha, N. Gajanana Kustagi, K.R. Lingamurthy, N. Ashoka and Nagaraju
College of Horticulture, Munirabad-583233, Koppal, Karnataka
*Corresponding author
Abstract:

The present study was carried out to know the sap transmission nature of sunflower necrosis virus and detection by serological and molecular means. The disease was successfully transmitted through mechanical sap using 0.05 M potassium phosphate buffer. A maximum transmission of 52.33 per cent on cultivar KBSH-44, 51.33 per cent on KBSH-1, and 48.33 per cent on Morden. Under artificial inoculation, the newly emerged leaves showed slight downward curling, puckering and chlorosis followed by necrosis. The sunflower genotypes subjected for serological detection by using DAC-ELISA a positive reaction with Tobacco Streak Virus (TSV) antisera was obtained, further, The RT-PCR was performed using RKJ primers specific for the coat protein gene of TSV and resulted in an amplicon of approximately 700bp. These results thus prove the transmissible nature of SNV through mechanical sap.


Keywords: Sunflower necrosis virus, Tobacco streak virus and sap inoculation
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How to cite this article:

Ravi Kumar, B. Jagadeesha, N. Gajanana Kustagi, K.R. Lingamurthy, N. Ashoka and Nagaraju. 2019. Paddy Crop Status in Tamil Nadu – A Statistical Analysis.Int.J.Curr.Microbiol.App.Sci. 8(2): 3203-3208. doi: https://doi.org/10.20546/ijcmas.2019.802.374