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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 3010-3018
DOI: https://doi.org/10.20546/ijcmas.2019.802.352


A Comparative Study of Peripheral Blood Smear and RDTs in Falciparum Malaria Diagnosis
C.M. Swathi1* and Meera Bai2
1Department of Microbiology, Mallareddy Medical College for Women and Mallareddy Narayana Multispeciality Hospital, Hyderabad, Telangana, India
2Department of microbiology, SRRITCD, Nallakunta, Hyderabad, Telangana, India
*Corresponding author
Abstract:

The present study was aimed to do comparative evaluation of efficacy of various staining techniques and rapid diagnostic tests used for identifying the Plasmodium falciparum parasite, to determine sensitivity and specificity of rapid diagnostic methods and compare the results of rapid diagnostic methods with conventional microscopy. Total of 107 male and female febrile patients of all age, clinically suspected to be positive for malaria, willing to participate and to sign the informed consent form were included in this study. Smears were stained by Leishman, Geimsa and JSB and antigen detection is done b by rapid test. Among the 107 clinically suspected cases, Leishman stain detected 18(16.8%) P.falciparum. Geimsa stain detected 16(14.9%) P.falciparum. JSB stain detected 15(14%) P.falciparum. RDT was positive for P.falciparum 18(16.8%) cases. In comparison to Leishman stain as gold standard the sensitivity, specificity, PPV and NPV of Geimsa, JSB and RDTs for P. falciparum were 88.8%, 100%, 100%, 97.8%, 83.3%, 100%, 100%, 96.7%, 55.5%, 91%, 55.5% and 91% respectively. Antigen based (HRP-2) RDTs are as specific as conventional microscopy but less sensitive. Their performance is highly affected by parasite density calling for further evaluation before incorporating them as diagnostic tools in peripheral health service. It is also understood that RDTs can be used only in conjunction with microscopy to improve the diagnosis of malaria.


Keywords: The present study was aimed to do comparative evaluation of efficacy of various staining techniques and rapid diagnostic tests used for identifying the Plasmodium falciparum parasite, to determine sensitivity and specificity of rapid diagnostic methods an
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How to cite this article:

Swathi, C.M., and Meera Bai. 2019. A Comparative Study of Peripheral Blood Smear and RDTs in Falciparum Malaria Diagnosis.Int.J.Curr.Microbiol.App.Sci. 8(2): 3010-3018. doi: https://doi.org/10.20546/ijcmas.2019.802.352