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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 2763-2771
DOI: https://doi.org/10.20546/ijcmas.2019.802.324


Determination of Tetracycline Residues in Milk by High Performance Liquid Chromatography
Sneh Lata Chauhan, Priyanka, S.R. Garg and Vijay J. Jadhav*
Department of Veterinary Public Health & Epidemiology, College of Veterinary Sciences LUVAS, Hisar, Haryana- 125004, India
*Corresponding author
Abstract:

A Reverse phase high-performance liquid chromatographic method (RP-HPLC) for the simultaneous determination of tetracycline, Oxytetracycline and chlortetracycline residues in milk has been developed. The determination of these antimicrobials was carried out using HPLC UV-VIS with a C8 hybrid column elution with a mobile phase composed of solvent A (water: formic acid as 1000:1 v/v) and solvent B (water: acetonitrile: formic acid as 100:900:1). Sample preparation involved protein precipitation followed by solid-phase extraction using a C18 cartridge. The method was validated and applied for the analysis of different type of milk samples commercialized in Hisar, Haryana. The limits of quantitation for all antimicrobials were below the maximum residue limit, which indicates that the method is appropriate for the determination of these antimicrobials in milk.


Keywords: Tetracycline residues, RP-HPLC, Milk, Solid phase extraction
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How to cite this article:

Sneh Lata Chauhan, Priyanka S.R. Garg and Vijay J. Jadhav. 2019. Determination of Tetracycline Residues in Milk by High Performance Liquid Chromatography.Int.J.Curr.Microbiol.App.Sci. 8(2): 2763-2771. doi: https://doi.org/10.20546/ijcmas.2019.802.324