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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2017: 100.00
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2017: 100.00
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2017: 100.00
NAAS RATING 2018: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(2): 2666-2681
DOI: https://doi.org/10.20546/ijcmas.2019.802.312


PCR Based Molecular Diagnostic Assays for Brucellosis: A Review
Vinay Kumar*, Nitish Bansal, Trilok Nanda, Aman Kumar, Rajni Kumari and Sushila Maan
Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar – 125004, India
*Corresponding author
Abstract:

Brucellosis is a worldwide re-emerging zoonotic disease of public health and economic importance. It affects a large number of domestic as well as wild animals and results in heavy losses to the animal husbandry sector. The direct culture of bacteria and serological test are the gold standard for Brucella spp. identification in the clinical samples. However, these assays have various limitations therefore PCR can be a potential tool to address aforesaid limitations and can be used for early detection of causative agents in disease condition. In this review, we have tried to discuss most of the currently used PCR based methods for detection of Brucella at genus and species level in different biological samples. Now a day, these assays are becoming very important tools for the identification of Brucella at genus, species and biovar level.


Keywords: Brucellosis, PCR based Molecular diagnostic assays
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How to cite this article:

Vinay Kumar, Nitish Bansal, Trilok Nanda, Aman Kumar, Rajni Kumari and Sushila Maan. 2019. PCR Based Molecular Diagnostic Assays for Brucellosis: A Review.Int.J.Curr.Microbiol.App.Sci. 8(2): 2666-2681. doi: https://doi.org/10.20546/ijcmas.2019.802.312