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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 398-414
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.044


Conquer Fluoroquinolone Multi-drug Resistant Salmonella enterica: Based on Biological Synthesis of Silver Nanoparticles using Citrus sinesis Peel Extract as an Alternative Therapeutic Pathway
Neveen M. Saleh1* and Mohamed S. Attia2,3
1Microbiology Department, National Organization for Drug Control and
Research (NODCAR), Giza, Egypt
2Chemistry Department, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia
3Chemistry Department, Faculty of Science, Ain Shams University, Abbassia, Cairo, Egypt
*Corresponding author
Abstract:

Antibiotic resistance is a worldwide health problem increasing with developing resistant strains. Therefore, there is a growing need to develop a simple, economic, commercially viable as well as the eco-friendly clean route for the synthesis of nanoparticles that can be used as nanodrug against multidrug resistance pathogens. To achieve this goal, peel extract of Citrus sinensis was implemented for nanoparticles synthesis in this study. The successful formation of silver nanoparticles has been monitored by ultraviolet–visible spectroscopy. Biosynthesized nanoparticles were characterized using transmission electron microscopy and X-ray diffraction spectra respectively. Seventy-two clinical Salmonella sp. isolates were isolated and fluoroquinolone resistance mechanisms of five isolates were studied. Cell wall modification, over expression of efflux pump and gene mutation in gyrA and/or parC genes were revealed. In vitro bio Ag-NPs showed effective bactericidal activity against forty-five Salmonella sp. with 40 μg/mlas minimum inhibitory concentration. Growth kinetics of Salmonella typhimurium showed a reduction in the number of viable cells after 24 h. According to transmission electron microscope, cell wall was damaged by pit formation. A membrane with such morphology exhibited a significant (p< 0.05) increase in permeability with increasing leakage of cytoplasm constitutes as proteins and nucleic acid.


Keywords: Salmonella enteric,Antibiotic resistance, orange peels extract, Bio-AgNPs, Inhibition mechanism.
 
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How to cite this article:

Neveen M. Saleh and Mohamed S. Attia. 2016. Conquer Fluoroquinolone Multi-drug Resistant Salmonella enterica: Based on Biological Synthesis of Silver Nanoparticles using Citrus sinesis Peel Extract as an Alternative Therapeutic Pathway.Int.J.Curr.Microbiol.App.Sci. 5(12): 398-414. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.044